Structural determination of group A Streptococcal surface dehydrogenase and characterization of its interaction with urokinase-type plasminogen activator receptor
文献类型： 外文期刊
作者： Li, Rui 1 ; Liang, Chenghui 2 ; Jiang, Longguang 3 ; Yuan, Cai 2 ; Huang, Mingdong 3 ;
作者机构： 1.Henan Acad Agr Sci, Minist Agr, Henan Prov Key Lab Anim Immunol, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China
2.Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Fujian, Peoples R China
3.Fuzhou Univ, Coll Chem, Fuzhou 350116, Fujian, Peoples R China
关键词： Group A Streptococcus; Streptococcal surface dehydrogenase; Crystal structure; Urokinase-type plasminogen activator receptor; Interaction
期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）
ISSN： 0006-291X
年卷期： 2019 年 510 卷 4 期
页码：
收录情况： SCI
摘要： Streptococcus pyogenes (group A Streptococcus, GAS) has caused a wide variety of human diseases. Its multifunctional surface dehydrogenase (SDH) is crucial for GAS life cycle. Furthermore, GAS infection into human pharyngeal cells has been previously shown to be mediated by the interaction between SDH and host urokinase-type plasminogen activator receptor (uPAR). However, the structural information of SDH remains to be elucidated and there are few detailed studies to characterize its interaction with uPAR. In-depth research on these issues will provide potential targets and strategies for combating GAS. Here, we prepared recombinant SDH tetramer in Escherichia coli BL21 (DE3) cells. After purification and crystallization, we determined its crystal structure at 1.74 angstrom. The unique characteristics might be potentially explored as drug targets or vaccine immunogen. We subsequently performed gel filtration chromatography, native-polyacrylamide gel electrophoresis (PAGE) and in vitro pull-down analyses. The results showed that their interaction was too weak to form stable complexes and the role of uPAR involved in GAS infection needs further demonstration. Altogether the current work provides the first view of SDH and deepens the knowledge of GAS infection. (C) 2019 Elsevier Inc. All rights reserved.
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