PacBio full-length transcriptome analysis reveals the role of tRNA-like structures in RNA processing
文献类型： 外文期刊
作者： Hu, Yanping 1 ; Yan, Shuangyong 3 ; Yan, Haohao 1 ; Su, Jingping 3 ; Cui, Zhongqiu 3 ; Li, Junling 3 ; Wang, Shengjun 3 ; Sun, Yue 3 ; Li, Wenjing 2 ; Gao, Shan 1 ;
作者机构： 1.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resourc, Xining 810008, Qinghai, Peoples R China
3.Tianjin Acad Agr Sci, Inst Crop Res, Tianjin 300381, Peoples R China
关键词： TIS; TTS; polyA-like site; tRNA-like site; Inverted repeat
期刊名称：CELLULAR SIGNALLING （ 影响因子：3.7； 五年影响因子：4.2 ）
ISSN： 0898-6568
年卷期： 2025 年 125 卷
页码：
收录情况： SCI
摘要： Background: Mitochondrial DNA (mtDNA) and chloroplast DNA (cpDNA) are distinct from nuclear DNA (nuDNA) in a eukaryotic cell. Animal mitochondria transcribe a single primary transcript that carries all genes from a DNA strand; In contrast, plant mitochondria and chloroplasts produce multiple primary transcripts, with each transcript carrying several genes. How primary transcripts of plant mtDNA and cpDNA are processed into mature RNAs is still unknown. Results: In the present study, we employed PacBio's full-length transcriptome data to characterize the transcription of Arabidopsis thaliana mtDNA, providing a more comprehensive and precise understanding. The primary findings included 20 novel mitochondrial (mt) RNAs of A. thaliana, transcripts carrying single introns or exons, long mt and chloroplast (cp) tRNAs with intricate secondary structures, and the role of tRNA-like structures in RNA processing. The gene of No. 20 novel mt RNA and its paralog on chromosome 2 of A. thaliana were assigned locus IDs ATMG01335 and AT2G07811. Conclusions: According to our upgraded "mitochondrial cleavage" model, tRNA-like structures serve as "punctuation" marks for RNA processing, akin to the role of tRNAs. Both tRNA-like structures and tRNAs collaborate for RNA processing in plant mitochondria and chloroplasts.
- 相关文献
作者其他论文 更多>>
-
Water level regimes can regulate the influences of microplastic pollution on carbon loss in paddy soils: Insights from dissolved organic matter and carbon mineralization
作者：Lu, Xiaonan;Wang, Lili;Hu, Runan;Li, Tingxuan;Guggenberger, Georg;Sun, Yue
关键词：Dissolved organic carbon; Enzyme activities; Polyethylene; Microbial community; Microplastics; Soil organic carbon mineralization
-
Antagonistic control of rice immunity against distinct pathogens by the two transcription modules via salicylic acid and jasmonic acid pathways
作者：Zhu, Xiaoying;Zhao, Yudan;Shi, Cheng-Min;Xu, Guojuan;Wang, Nana;Ning, Yuese;Kang, Houxiang;Liu, Wende;Wang, Ruyi;Wang, Xuli;Shi, Cheng-Min;Zuo, Shimin;Yan, Shuangyong;Wang, Guo-Liang
关键词：
-
Rice-Magnaporthe oryzae interactions in resistant and susceptible rice cultivars under panicle blast infection based on defense-related enzyme activities and metabolomics
作者：Yang, Xiurong;Li, Guangsheng;Li, Yuejiao;Sun, Shuqin;Huo, Jianfei;Yan, Shuangyong;Li, Junling;Cui, Zhongqiu;Sun, Yue
关键词：
-
OsIPK1 frameshift mutations disturb phosphorus homeostasis and impair starch synthesis during grain filling in rice
作者：Wang, Lina;Cui, Jing;Zhang, Ning;Wang, Xueqin;Wu, Shian;Yao, Wei;Chen, Defu;Chen, Xiwen;Su, Jingping;Valles, Maria Pilar;Chen, Defu
关键词：OsIPK1; Phytic acid; Homozygous lethality; Starch synthesis; Rice
-
Identification of unique ecosystem service bundles in farmland - A case study in the Huang-Huai-Hai Plain of China
作者：Gong, Lingxuan;Wang, Lili;Hu, Runan;Lu, Xiaonan;Zhang, Siqi;Zhang, Guilong;Tan, Bingchang;Sun, Yue
关键词：Ecosystem service multifunctionality; Trade-offs/synergies; Ecosystem service bundles; InVEST model; The Huang-Huai-Hai Plain of China
-
Defense-Related Enzyme Activities and Metabolomic Analysis Reveal Differentially Accumulated Metabolites and Response Pathways for Sheath Blight Resistance in Rice
作者：Yang, Xiurong;Li, Yuejiao;Li, Guangsheng;Zhao, Yujiao;Sun, Shuqin;Huo, Jianfei;Yan, Shuangyong;Su, Jingping;Cui, Zhongqiu;Sun, Yue;Yi, Heng;Wang, Shengjun;Zhao, Yujiao;Li, Zhibin
关键词：rice sheath blight; rice cultivar; defense enzyme; disease resistance; untargeted metabolomics
-
Discovery of the first Tn630 member and the closest homolog of IS630 from viruses
作者：Hu, Yanping;Li, Wenjing;Yan, Haohao;Guo, Yutong;Chang, Jia;Gao, Shan;Duan, Guangyou;Zhou, Mingbing;Yan, Shuangyong;Ruan, Cihan
关键词：TE; ITm; Mariner; Pogo; HGT
