Enhancing pest control in pear orchards: Harnessing nanocarrier SPc for efficient delivery of emamectin benzoate and dslgl to safeguard beneficial insects
文献类型： 外文期刊
作者： Li, Jianying 1 ; Zhang, Dongyue 1 ; Wang, Yilin 1 ; Xing, Kun 3 ; Zhao, Fei 3 ; Li, Zhen 1 ; Liu, Xiaoxia 1 ; Zhang, Songdou 1 ;
作者机构： 1.China Agr Univ, Coll Plant Protect, Dept Entomol, Beijing 100193, Peoples R China
2.China Agr Univ, Coll Plant Protect, MOA Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China
3.Shanxi Agr Univ, Coll Plant Protect, Shanxi Key Lab Integrated Pest Management Agr, Taiyuan 030031, Peoples R China
4.China Agr Univ, Sanya Inst, Sanya City 572025, Hainan, Peoples R China
关键词： Insecticide; Lethal giant larvae (lgl) gene; Grapholitha molesta; Cacopsylla chinensis; biopesticide
期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：4.6； 五年影响因子：4.4 ）
ISSN： 0171-8177
年卷期： 2025 年 45 卷 1 期
页码：
收录情况： SCI
摘要： Chemical control stands as a cornerstone in orchard pest management, yet concerns arise from prolonged usage including resistance development, non-target organism risks, and environmental repercussions. Recent advancements leveraging nanocarriers for co-delivery of insecticides and dsRNA present a promising, efficient, and environmentally sound approach to pest control. In this study, we harnessed SPc for the simultaneous delivery of emamectin benzoate (EB) and dsRNA targeting lethal giant larvae (lgl) gene to combat two major orchard pests, Grapholitha molesta and Cacopsylla chinensis. This innovative strategy not only bolstered insecticide efficacy but also addressed resistance challenges and safety considerations. Initially, we assessed the efficacy of EB across various life stages of the pests. Subsequent RNA-seq analysis unveiled lgl gene upregulation following EB treatment, with lgl gene silencing heightening pest susceptibility to EB. By utilizing the pET30a-BL21-RNase III- system to express hairpin RNA hplgl, we engineered a ternary biopesticide (hplgl-SPc-EB complex). This biopesticide exhibited remarkable control over G. molesta and C. chinensis, achieving substantial mortality rates at minimal EB doses. Notably, the ternary biopesticide posed no detrimental impacts on natural enemies, enabling seamless integration with biological control methods. Our findings highlight the advantages of ternary biopesticides in reducing insecticide reliance, promoting environmental sustainability, managing resistance, and offering a comprehensive strategy for orchard pest management.
- 相关文献
作者其他论文 更多>>
-
Heatwave types and frequency alter multigenerational ecological response of wheat aphids
作者：Cao, Junyu;Xing, Kun;Zhao, Fei;Li, Weiwei
关键词：
Sitobion avenae ; Heat stress; Maternal; Offspring; Fitness traits -
Multigenerational responses of wheat aphids to multiple stresses: interactions between sublethal thermal stress types and a low insecticide dose
作者：Xing, Kun;Mo, Ze-Fang;Xu, Zhi-Ping;Wei, Jia-jia;Cheng, Hui;Zhao, Fei;Xing, Kun;Mo, Ze-Fang;Xu, Zhi-Ping;Wei, Jia-jia;Cheng, Hui;Zhao, Fei
关键词：climate change; delayed effects; imidacloprid; stress interactions
-
Complete mitochondrial genome of Rhopalosiphum maidis (Hemiptera: Aphididae) and its phylogenetic implications
作者：Zhang, Miao;Xing, Kun;Zhao, Fei;Kang, Chen
关键词：Rhopalosiphum maidis; phylogenetic tree; mitogenome
-
Response of wheat aphid to insecticides is influenced by the interaction between temperature amplitudes and insecticide characteristics
作者：Xing, Kun;Zhang, Shu-Ming;Jia, Mei-Qi;Zhao, Fei;Xing, Kun;Zhang, Shu-Ming;Jia, Mei-Qi;Zhao, Fei
关键词：global warming; insecticides; multiple stress; risk assessment; temperature amplitude; temperature characteristics
-
Acclimation Effects of Natural Daily Temperature Variation on Longevity, Fecundity, and Thermal Tolerance of the Diamondback Moth (Plutella xylostella)
作者：Xing, Kun;Zhao, Fei
关键词：thermal acclimation; temperature amplitude; life history trait; thermal tolerance
-
The complete mitochondrial genome of Lixus subtilis Boheman, 1835 (Coleoptera, Curculionidae) and its phylogenetic implications
作者：Xing, Kun;Chen, Kang;Zhao, Xiao-jun;Zhao, Fei
关键词：Lixus subtilis; mitochondrial genome; phylogeny
-
Complex delayed and transgenerational effects driven by the interaction of heat and insecticide in the maternal generation of the wheat aphid, Sitobion avenae
作者：Cao, Jun-Yu;Cao, Jun-Yu;Xing, Kun;Zhao, Fei;Xing, Kun;Zhao, Fei
关键词：development; thermal intensity; imidacloprid; fitness; progeny
