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Identification of harmful protozoa in outdoor cultivation of Chlorella and the use of ultrasonication to control contamination

文献类型： 外文期刊

作者： Wang, Yao 1 ; Gong, Yingchun 2 ; Dai, Lili 7 ; Sommerfeld, Milton 4 ; Zhang, Chengwu 1 ; Hu, Qiang 2 ;

作者机构： 1.Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China

2.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China

3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China

4.Arizona State Univ, Coll Technol & Innovat, Lab Algae Res & Biotechnol, Mesa, AZ 85212 USA

5.China Elect Engn Design Inst, SDIC Microalgae Biotechnol Ctr, Beijing 100142, Peoples R China

6.Beijing Key Lab Algae Biomass, Beijing 100142, Peoples R China

7.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Hubei, Peoples R China

关键词： Contamination control; Chlorella sp.; Poterioochromonas sp.; Identification; Ultrasonication; DGGE

期刊名称：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS （ 影响因子：4.401； 五年影响因子：5.278 ）

ISSN： 2211-9264

年卷期： 2018 年 31 卷

页码：

收录情况： SCI

摘要： Contaminating organisms in mass cultivation present one of the major challenges that must be overcome for successful commercialization of algal biofuels. The present study identified a range of contaminating organisms in Chlorella cultures cultivated in outdoor raceway ponds at the Arizona Center for Algae Technology and Innovation (Mesa, AZ). Nineteen organisms or Operational Taxonomic Units (OTU) in the Chlorella culture were identified by a combination of microscopic observation and 18S rRNA denaturing gradient gel electrophoresis (DGGE). More detailed analyses identified these contaminating organisms as 2 fungi, 7 flagellates, 3 amoebae, 4 ciliates, 1 rotifer, and 2 large insects. Among them Poterioochromonas sp., a small flagellate, appeared to be one of the most harmful causing culture collapse. In order to control Poterioochromonas sp., various operational parameters and application strategies of ultrasonic treatment were investigated. During sixteen-day consecutive Chlorella cultivation in batch mode, the ultrasonication conditions of 6 L min(-1) flow rate with the power of 495 W at 100% amplitude and a treatment frequency of once for 1 h every day, was proven to be the most effective in preventing Poterioochromonas outbreak in Chlorella culture with volume of 60 L. The above ultrasonication method was also effective at destroying an unknown fungus, an amoeba (Acanthocystis sp.), and ciliates (a member of the family Orchitophryidae). Our findings can serve as a technical foundation for the application of ultrasonication to control some of the contaminating microorganisms in mass cultivation of microalgae including Chlorella.

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