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De novo sequencing, assembly, and analysis of Iris lactea var. chinensis roots' transcriptome in response to salt stress

文献类型： 外文期刊

作者： Gu, Chunsun 1 ; Xu, Sheng 1 ; Wang, Zhiquan 1 ; Liu, Liangqin 1 ; Zhang, Yongxia 1 ; Deng, Yanming 3 ; Huang, Suzhen 1 ;

作者机构： 1.Inst Bot, Jiangsu Prov Platform Conservat & Utilizat Agr Ge, Jiangsu Key Lab Bioresources Saline Solis, Nanjing 210014, Jiangsu, Peoples R China

2.Chinese Acad Sci, Nanjing 210014, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Leisure Agr, Nanjing 210014, Jiangsu, Peoples R China

关键词： Transcriptome; Iris lactea var. chinensis; Halophyte; Salt stress; qRT-PCR

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2018 年 125 卷

页码：

收录情况： SCI

摘要： As a halophyte, Iris lactea var. chinensis (I. lactea var. chinensis) is widely distributed and has good drought and heavy metal resistance. Moreover, it is an excellent ornamental plant. I. lactea var. chinensis has extensive application prospects owing to the global impacts of salinization. To better understand its molecular mechanism involved in salt resistance, the de novo sequencing, assembly, and analysis of I. lactea var. chinensis roots' transcriptome in response to salt-stress conditions was performed. On average, 74.17% of the clean reads were mapped to unigenes. A total of 121,093 unigenes were constructed and 56,398 (46.57%) were annotated. Among these, 13,522 differentially expressed genes (DEGs) were identified between salt-treated and control samples Compared to the transcriptional level of control, 7037 DEGs were up-regulated and 6539 down-regulated. In addition, 129 up-regulated and 1609 down-regulated genes were simultaneously detected in all three pairwise comparisons between control and salt-stressed libraries. At least 247 and 250 DEGs encoding transcription factors and transporter proteins were identified. Meanwhile, 130 DEGs regarding reactive oxygen species (ROS) scavenging system were also summarized. Based on real-time quantitative RT-PCR, we verified the changes in the expression patterns of 10 unigenes. Our study identified potential salt-responsive candidate genes and increased the understanding of halophyte responses to salinity stress.

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