Transcriptome analysis of Hevea brasiliensis in response to exogenous methyl jasmonate provides novel insights into regulation of jasmonate-elicited rubber biosynthesis
文献类型： 外文期刊
作者： Liu, Jin-Ping 1 ; Hu, Jin 1 ; Liu, Yan-Hui 2 ; Yang, Cui-Ping 1 ; Zhuang, Yu-Fen 1 ; Guo, Xiu-Li 1 ; Li, Yi-Jian 3 ; Zhan 1 ;
作者机构： 1.Hainan Univ, Trop Agr & Forestry Inst, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou 570228, Hainan, Peoples R China
2.Fujian Agr & Forestry Univ, Ctr Genom & Biotechnol, Key Lab Minist Educ Genet Breeding & Multiple Uti, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Fuzhou 350002, Fujian, Peoples R China
3.Chinese Acad Trop Agr Sci, Rubber Res Inst, Serv Ctr Sci & Technol, Danzhou 571737, Hainan, Peoples R China
关键词： Rubber tree; Methyl jasmonate; Rubber biosynthesis; RNA sequencing; Transcriptome
期刊名称：PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS （ 影响因子：2.391； 五年影响因子：2.836 ）
ISSN： 0971-5894
年卷期： 2018 年 24 卷 3 期
页码：
收录情况： SCI
摘要： The phytohomorne methyl jasmonate (MeJA) is known to trigger extensive reprogramming of gene expression leading to transcriptional activation of many secondary metabolic pathways. However, natural rubber is a commercially important secondary metabolite and little is known about the genetic and genomic basis of jasmonate-elicited rubber biosynthesis in rubber tree (Hevea brasiliensis). RNA sequencing (RNA-seq) of H. brasiliensis bark treated with 1 g lanolin paste containing 0.02% w/w MeJA for 24 h (M2) and 0.04% w/w MeJA for 24 h (M4) was performed. A total of 2950 and 2850 differentially expressed genes in M2 and M4 compared with control (C) were respectively detected. Key genes involved in 2-C-methyl-D-erythritol 4-phosphate, rubber biosynthesis, glycolysis and carbon fixation (Calvin cycle) pathway were found to be up-regulated by MeJA treatment. Particularly, the expression of 3-hydroxy-3-metylglutaryl coenzyme A reductase in MVA pathway was down-regulated by MeJA treatment, but the expression of farnesyl diphosphate synthase (FPS) and cis-prenyltransferase (CPT, or rubber transferase) in rubber biosynthesis pathway were up-regulated by MeJA treatment. Up-regulation of critical genes in JA biosynthesis in response to MeJA treatment exhibited the self-activation of JA biosynthesis. In addition, up-regulated genes of great regulatory importance in cross-talk between JA and other hormone signaling, and of transcriptional regulation were identified. The increased expression levels of FPS and CPT in rubber biosynthesis pathway possibly resulted in an increased latex production in rubber tree treated with MeJA. The present results provide insights into the mechanism by which MeJA activates the rubber biosynthesis and the transcriptome data can also serve as the foundation for future research into the molecular basis for MeJA regulation of other cellular processes.
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