Physiological and transcriptomic analysis revealed the involvement of crucial factors in heat stress response of Rhododendron hainanense
文献类型： 外文期刊
作者： Zhao, Ying 1 ; Yu, Wengang 1 ; Hu, Xiangyu 1 ; Shi, Youhai 1 ; Liu, Yu 1 ; Zhong, Yunfang 1 ; Wang, Peng 2 ; Deng, Shuya; 1 ;
作者机构： 1.Hainan Univ, Inst Trop Agr & Forestry, Res Ctr Terr Biodivers South China Sea, Haikou 570228, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Minist Agr, Key Lab Crop Gene Resources & Germplasm Enhanceme, Trop Crops Genet Resources Inst, Danzhou 571737, Hainan, Peoples R China
关键词： Physiological analysis; Transcriptomic analysis; Transcription factors; Heat stress; Rhododendron hainanense
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
ISSN： 0378-1119
年卷期： 2018 年 660 卷
页码：
收录情况： SCI
摘要： Molecular regulatory mechanism of heat stress response (HSR) in Ericaceae remains unknown. Here, we sought to identify HSR mechanisms in Rhododendron hainanense, a Ericaceae species, through a combination of physiological and transcriptomic studies. The levels of MDA, H2O2, Pro, SOD, CAT and APX in leaves of R. hainanense were analyzed to characterize a dramatic difference in varied temperature treatment. Also, three sequencing libraries, including one control and two heat stress (HS)-treated samples, were constructed for comparative transcriptomic analysis. By Illumina sequencing and Trinity strategy, 350 million clean reads (average length = 149 bp) was assembled into 183,486 unigenes. According to analysis of differential expression genes (DEGs), a total of 2658 DEGs were obtained. Moreover, a complex interaction network of 982 DEGs was established, of which master portions were comprised of 109 transcription factors (TFs). Importantly, integrated differential expression profiling, qRT-PCR and functional analysis, several TFs of R. hainanense (ABR1, IAA26, OBF1, LUX, SCL3, DIV, NAC29, NAC72 and TCP3) and their potential regulations for the crosstalk between hormonal signal and HSR were identified. These findings will contribute to our understanding of the regulatory mechanisms of HSR in R. hainanense, breeding cultivars with improved thermotolerance.
- 相关文献
作者其他论文 更多>>
-
Systematic characterization of the bZIP gene family in Colletotrichum siamense and functional analysis of three family members
作者：Yang, Hong;Liu, Yu;Song, Miao;Wu, Wei;Lin, Chunhua;Yang, Hong;Huang, Weiyuan;Fan, Songle;Xue, Wenxuan;He, Qiguang;Wang, Li Feng;Huang, Weiyuan;Xue, Wenxuan
关键词：Colletotrichum siamense; bZIP transcription factors; Genome-wide identification fungicide; resistance; Virulence
-
Genome-Enabled Analyses Reveal Roles of Mangrove Homeobox Superfamily in Response to Salinity
作者：Chen, Tingting;Chen, Tingting;Ye, Xiuxu;Wang, Peng;Feng, Erhui;Zhang, Yu;Wang, Peng
关键词：Homeobox superfamily; Rhizophoraceae; Mangrove; Salinity stress
-
Discovery of ganodecalone A, from Ganoderma calidophilum for the treatment of acute gastric ulcers by binding to ALOX5 via the MAPK/ NF-κB/iNOS signaling
作者：Liu, Yu;Li, Weikang;Tong, Zhao;Luo, Duqiang;Liu, Yu;Li, Weikang;Tong, Zhao;Luo, Duqiang;Yang, Li;Zhao, Youxing;Wang, Zhen
关键词：Gastric ulcer; Ganoderma calidophilum; Ganodecalone A; Oxidative stress; Inflammation; MAPK/NF-kappa B/iNOS signalling
-
Comparative and Phylogenetic Analysis of Complete Chloroplast Genomes of Five Mangifera Species
作者：Tang, Yujuan;Yang, Xiangyan;Luo, Shixing;Huang, Guodi;Zhang, Yu;Zhao, Ying;Li, Riwang;Guo, Limei;Ran, Mengyang;Tang, Yujuan;Yang, Xiangyan;Luo, Shixing;Huang, Guodi;Zhang, Yu;Zhao, Ying;Li, Riwang;Guo, Limei;Ran, Mengyang;Tang, Yujuan;Yang, Xiangyan;Luo, Shixing;Huang, Guodi;Zhang, Yu;Zhao, Ying;Li, Riwang;Guo, Limei;Ran, Mengyang;Gao, Aiping;Huang, Jianfeng
关键词：Mangifera; chloroplast genome; Illumina; polymorphism analysis; phylogenetic analysis
-
PbrSYP71 regulates pollen tube growth by maintaining polar distribution of the endoplasmic reticulum in Pyrus
作者：Zhang, Mingliang;Tang, Chao;Wang, Zhiqi;Lan, Chi;Yue, Dong;Zhang, Ningyi;Xie, Zhihua;Qian, Ming;Sun, Mengjun;Liu, Zongqi;Xie, Zhu;Zhang, Hao;Zhang, Shaoling;Wang, Peng;Wu, Juyou;Zhang, Mingliang;Liu, Zhuqin;Wu, Juyou
关键词：endoplasmic reticulum; F-actin; PbrSYP71; pear (
Pyrus bretschneideri ); pollen tube; syntaxin -
Combined Analysis of the Leaf Metabolome, Lipidome, and Candidate Gene Function: Insights into Genotypic Variation in Phosphorus Utilization Efficiency in Stylosanthes guianensis
作者：Lv, Jinhui;Xu, Ranran;Zhang, Jianyu;Liu, Yu;Luo, Lijuan;Liu, Guodao;Liu, Pandao;Lv, Jinhui;Xu, Ranran;Zhang, Jianyu;Liu, Yu;Luo, Lijuan;Liu, Guodao;Liu, Pandao;Lv, Jinhui;Huang, Rui;Xu, Ranran;Zhang, Jianyu;Liu, Yu;Liu, Guodao;Liu, Pandao;Wu, Yuanhang
关键词：phosphorus utilization efficiency; membrane lipid remodeling; purple acid phosphatase;
Stylosanthes guianensis ; pioneer plant -
Numerical simulation and optimisation of depodding performance in a fresh green soybean harvester
作者：Zhao, Ying;Yang, Ranbing;Zhang, Xirui;Liu, Jinyi;Lin, Mao;Xing, Jiejie;Yang, Songmei;Hu, Can;Xi, Xiaobo;Ding, Wenqin;Xiao, Suwei
关键词：EDEM simulation; Breakage rate; Brushing and combing; Field verification experiment
