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Development of Species-Specific SCAR Markers, Based on a SCoT Analysis, to Authenticate Physalis (Solanaceae) Species

文献类型： 外文期刊

作者： Feng, Shangguo 1 ; Zhu, Yujia 1 ; Yu, Chenliang 3 ; Jiao, Kaili 1 ; Jiang, Mengying 1 ; Lu, Jiangjie 1 ; Shen, Chenjia 1 ;

作者机构： 1.Hangzhou Normal Univ, Coll Life & Environm Sci, Zhejiang Prov Key Lab Genet Improvement & Qual Co, Hangzhou, Zhejiang, Peoples R China

2.Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Vegetable, Hangzhou, Zhejiang, Peoples R China

关键词： Physalis species; species-specific; SCoT markers; SCAR markers; marker development

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN： 1664-8021

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： Physalis is an important genus in the Solanaceae family. It includes many species of significant medicinal value, edible value, and ornamental value. However, many Physalis species are easily confused because of their similar morphological traits, which hinder the utilization and protection of Physalis resources. Therefore, it is necessary to create fast, sensitive, and reliable methods for the Physalis species authentication. Intended for that, in this study, species-specific sequence-characterized amplified region (SCAR) markers were developed for accurate identification of the closely related Physalis species P. angulata, P. minima, P. pubescens, and P. alkekengi var. franchetii, based on a simple and novel marker system, start codon targeted (SCoT) marker. A total of 34 selected SCoT primers yielded 289 reliable SCoT loci, of which 265 were polymorphic. Four species-specific SCoT fragments (SCoT3-1404, SCoT3-1589, SCoT5-550, and SCoT36-520) from Physalis species were successfully identified, cloned, and sequenced. Based on these selected specific DNA fragments, four SCAR primers pairs were developed and named ST3KZ, ST3MSJ, ST5SJ, and ST36XSJ. PCR analysis of each of these primer pairs clearly demonstrated a specific amplified band in all samples of the target Physalis species, but no amplification was observed in other Physalis species. Therefore, the species-specific SCAR primer pairs developed in this study could be used as powerful tools that can rapidly, effectively, and reliably identify and differentiate Physalis species.

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