文献类型： 外文期刊

作者： Lu, Gui-Hua ¹ ; Hua, Xiao-Mei ³ ; Liang, Li ¹ ; Wen, Zhong-Ling ¹ ; Du, Mei-Hang ¹ ; Meng, Fan-Fan ⁴ ; Pang, Yan-Jun ¹ ;

作者机构： 1.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Inst Plant Mol Biol, Nanjing 210023, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Modem Crop Prod, Nanjing 210095, Jiangsu, Peoples R China

3.Nanjing Inst Environm Sci, MEP, State Environm Protect Key Lab Soil Environm Mana, Nanjing 210042, Jiangsu, Peoples R China

4.Jilin Acad Agr Sci, Changchun 130033, Jilin, Peoples R China

关键词： soil; rhizosphere; glyphosate-tolerant soybean; 16S rRNA gene; plant growth-promoting rhizobacteria; shotgun metagenomic approach

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN： 2073-4425

年卷期： 2018 年 9 卷 4 期

页码：

收录情况： SCI

摘要： The worldwide commercial cultivation of transgenic crops, including glyphosate-tolerant (GT) soybeans, has increased widely during the past 20 years. However, it is accompanied with a growing concern about potential effects of transgenic crops on the soil microbial communities, especially on rhizosphere bacterial communities. Our previous study found that the GT soybean line NZL06-698 (N698) significantly affected rhizosphere bacteria, including some unidentified taxa, through 16S rRNA gene (16S rDNA) V4 region amplicon deep sequencing via Illumina MiSeq. In this study, we performed 16S rDNA V5-V7 region amplicon deep sequencing via Illumina MiSeq and shotgun metagenomic approaches to identify those major taxa. Results of these processes revealed that the species richness and evenness increased in the rhizosphere bacterial communities of N698, the beta diversity of the rhizosphere bacterial communities of N698 was affected, and that certain dominant bacterial phyla and genera were related to N698 compared with its control cultivar Mengdou12. Consistent with our previous findings, this study showed that N698 affects the rhizosphere bacterial communities. In specific, N698 negatively affects Rahnella, Janthinobacterium, Stenotrophomonas, Sphingomonas and Luteibacter while positively affecting Arthrobacter, Bradyrhizobium, Ramlibacter and Nitrospira.