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Mining for genes encoding proteins associated with NopL of Sinorhizobium fredii HH103 using quantitative trait loci in soybean (Glycine max Merr.) recombinant inbred lines

文献类型： 外文期刊

作者： Zhang, Yanjiao 1 ; Liu, Xueying 1 ; Chen, Lin 1 ; Fu, Ying 1 ; Li, Changyu 1 ; Qi, Zhaoming 1 ; Zou, Jianan 1 ; Zhu, Rong 1 ;

作者机构： 1.Northeast Agr Univ, Chinese Minist Educ, Key Lab Soybean Biol & Breeding Genet,Chinese Agr, Key Lab Soybean Biol,Coll Sci, Harbin, Heilongjiang, Peoples R China

2.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

3.Fudan Univ, Sch Life Sci, Inst Plant Biol, Collaborat Innovat Ctr Genet & Dev, Shanghai 200433, Peoples R China

4.Heilongjiang Acad Agr Sci, Suihua Branch, Suihua, Peoples R China

5.Heilongjiang Acad Agr Sci, Crop Breeding Inst, Harbin, Heilongjiang, Peoples R China

关键词： Soybean; NopL; Symbiosis; QTL

期刊名称：PLANT AND SOIL （ 影响因子：4.192； 五年影响因子：4.712 ）

ISSN： 0032-079X

年卷期： 2018 年 431 卷 1-2 期

页码：

收录情况： SCI

摘要： In rhizobia, host specificity is controlled by the nodulation outer proteins (Nops). However, the host genes encoding proteins associated with Nops remain unknown. We constructed a Sinorhizobium fredii HH103 Omega nopL mutant and analyzed the nodule number (NN) and nodule dry weight (NDW) of 10 germplasms inoculated with the mutant or wild-type strain. Using recombinant inbred lines (RILs), the conditional and unconditional quantitative trait loci (QTLs) associated with NopL were detected. The regions overlapping conditional and unconditional QTLs were identified. Additionally, the expression levels of selected candidate genes were analyzed by qRT-PCR. Depending on the germplasm, NopL positively or negatively affected NN and NDW. The NN and NDW of 'Charleston' and 'Dongnong 594' (RIL parents) were lower in samples inoculated with Sinorhizobium fredii HH103 Omega nopL than in samples inoculated with the wild-type strain. In soybean inoculated independently with Sinorhizobium fredii HH103 and HH103 Omega nopL, 11 and 5 unconditional QTLs were identified for NN and NDW, respectively, while five and two conditional QTLs were identified for NN and NDW, respectively. Two overlapping QTLs were identified among the conditional and unconditional QTLs. Furthermore, the enzymes encoded by Glyma.13g170500 and Glyma.07g099700 likely interacted with NopL. The Glyma.13g170500 and Glyma.07g099700 detected at QTLs represent candidate genes that encode enzymes that interact with NopL to regulate the establishment of symbiotic relationships between rhizobia and soybean.

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