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A cytosolic NAD(+)-dependent GPDH from maize (ZmGPDH1) is involved in conferring salt and osmotic stress tolerance

文献类型： 外文期刊

作者： Zhao, Ying 1 ; Liu, Meng 1 ; He, Lin 1 ; Li, Xin 2 ; Wang, Feng 1 ; Yan, Bowei 1 ; Wei, Jinpeng 1 ; Zhao, Changjiang 1 ; Li, 1 ;

作者机构： 1.Heilongjiang Bayi Agr Univ, Coll Agr, Daqing Key Lab Straw Reclamat Technol Res & Dev, Key Lab Modern Agr Cultivat & Crop Germplasm Impr, Daqing 163319, Peoples R China

2.Heilongjiang Acad Agr Sci, Key Lab Maize Genet & Breeding, Harbin 150000, Heilongjiang, Peoples R China

关键词： Glycerol-3-phosphate dehydrogenase; Glycerol; Antioxidants; Redox homeostasis; Salt stress; Osmotic stress; Maize (Zea mays L; )

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2019 年 19 卷

页码：

收录情况： SCI

摘要： BackgroundPlant glycerol-3-phosphate dehydrogenase (GPDH) catalyzes the reduction of dihydroxyacetone phosphate (DHAP) to produce glycerol-3-phosphate (G-3-P), and plays a key role in glycerolipid metabolism as well as stress responses.ResultsIn this study, we report the cloning, enzymatic and physiological characterization of a cytosolic NAD(+)-dependent GPDH from maize. The prokaryotic expression of ZmGPDH1 in E.coli showed that the enzyme encoded by ZmGPDH1 was capable of catalyzing the reduction of DHAP in the presence of NADH. The functional complementation analysis revealed that ZmGPDH1 was able to restore the production of glycerol-3-phosphate and glycerol in AtGPDHc-deficient mutants. Furthermore, overexpression of ZmGPDH1 remarkably enhanced the tolerance of Arabidopsis to salinity/osmotic stress by enhancing the glycerol production, the antioxidant enzymes activities (SOD, CAT, APX) and by maintaining the cellular redox homeostasis (NADH/NAD(+), ASA/DHA, GSH/GSSG). ZmGPDH1 OE Arabidopsis plants also exhibited reduced leaf water loss and stomatal aperture under salt and osmotic stresses. Quantitative real-time RT-PCR analyses revealed that overexpression of ZmGPDH1 promoted the transcripts accumulation of genes involved in cellular redox homeostasis and ROS-scavenging system.ConclusionsTogether, these data suggested that ZmGPDH1 is involved in conferring salinity and osmotic tolerance in Arabidopsis through modulation of glycerol synthesis, stomatal closure, cellular redox and ROS homeostasis.

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