文献类型： 外文期刊

作者： Zhao, Tingting ¹ ; Ren, Lijun ¹ ; Chen, Xiaojun ¹ ; Yu, Hengxiu ⁵ ; Liu, Chengjie ⁶ ; Shen, Yi ¹ ; Shi, Wenqing ¹ ; Tang, ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

2.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Plant Gene Res, Beijing 100101, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Ningxia Acad Agr & Forestry Sci, Key Lab Agr Biotechnol Ningxia, Ningxia 750002, Peoples R China

5.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Yangzhou 225009, Jiangsu, Peoples R China

6.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China

期刊名称：PLANT CELL （ 影响因子：11.277； 五年影响因子：12.061 ）

ISSN： 1040-4651

年卷期： 2018 年 30 卷 12 期

页码：

收录情况： SCI

摘要： Response regulators play significant roles in controlling various biological processes; however, their roles in plant meiosis remain unclear. Here, we report the identification of OsRR24/LEPTOTENE1 (LEPTO1), a rice (Oryza sativa) type-B response regulator that participates in the establishment of key molecular and morphological features of chromosomes in leptotene, an early stage of prophase I in meiosis. Although meiosis initiates normally, as indicated by staining of the centromere-specific histone CENH3, the meiotic chromosomes in lepto1 mutant pollen mother cells fail to form the thin thread-like structures that are typical of leptotene chromosomes in wild-type pollen mother cells. Furthermore, lepto1 mutants fail to form chromosomal double-strand breaks, do not recruit meiosis-specific proteins to the meiotic chromosomes, and show disrupted callose deposition. LEPTO1 also is essential for programmed cell death in tapetal cells. LEPTO1 contains a conserved signal receiver domain (DDK) and a myb-like DNA binding domain at the N terminus. LEPTO1 interacts with two authentic histidine phosphotransfer (AHP) proteins, OsAHP1 and OsAHP2, via the DDK domain, and a phosphomimetic mutation of the DDK domain relieves its repression of LEPTO1 transactivation activity. Collectively, our results show that OsRR24/LEPTO1 plays a significant role in the leptotene phase of meiotic prophase I.