Role of type IV secretion system genes in virulence of rice bacterial brown stripe pathogen Acidovorax oryzae strain RS-2
文献类型： 外文期刊
作者： Ogunyemi, Solabomi Olaitan 1 ; Fang, Yushi 1 ; Qiu, Wen 1 ; Li, Bin 1 ; Chen, Jie 1 ; Yang, Min 1 ; Hong, Xianxian 1 ; Lu 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects, State Key Lab Rice Biol,Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China
2.Fed Univ Agr Abeokuta, Dept Crop Protect, Abeokuta, Nigeria
3.Shanghai Extens & Serv Ctr Agr Technol, Dept Plant Quarantine, Shanghai 201103, Peoples R China
4.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Acidovorax oryzae; T4SS; Gene knock-out; Virulence; Biofilm; Motility
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）
ISSN： 0882-4010
年卷期： 2019 年 126 卷
页码：
收录情况： SCI
摘要： Type IV secretion system (T4SS) is a specialized nanomachine that is utilized for the pathogenicity of gram-negative bacteria. However, the role of T4SS genes in virulence of rice bacterial brown stripe pathogen Acidovorax oryzae (Ao) strain RS-2 is not clear, which contains T4SS gene cluster based on genome-wide analysis. Here we compared the virulence-related phenotypes between the wild-type strain RS-2 and nine T4SS mutants, which were constructed in this study. Results indicated that mutation of pilT, pilM, pilQ, or pilZ3 genes not only significantly reduced bacterial virulence, but also caused a reduction of 20.4-62.0% in biofilm formation and 37.7-47.7% reduction in motility, but had no effect on exopolysaccharide (EPS) production or extracellular enzymatic activities when compared to the wild type. The four T4SS genes had a differential effect on bacterial growth after 24 h post-incubation. The complemented strains of the four T4SS mutants restored similar virulence symptom as the wild type. In addition, no change was observed in bacterial virulence by mutation of the other five T4SS genes. Totally, these results demonstrated that T4SS played vital roles in bacterial virulence, motility and biofilm formation in plant pathogen Ao strain RS-2.
- 相关文献
作者其他论文 更多>>
-
Integrating RNA Interference and Nanotechnology: A Transformative Approach in Plant Protection
作者：Islam, Mohammad Shafiqul;Noman, Muhammad;Zhang, Zhen;Wang, Jing;Lu, Ziqi;Cai, Yingying;Wang, Yanli;Wang, Jiaoyu;Islam, Mohammad Shafiqul;Li, Bin;Islam, Mohammad Shafiqul;Li, Bin;Ahmed, Md Robel;Ahmed, Temoor;Ahmed, Temoor;Sarwar, Abul Khayer Mohammad Golam
关键词：fungal disease; nanotechnology; plant protection; plant-pathogen interactions; RNAi
-
Mitigating ethyl carbamate production in Chinese rice wine: Role of raspberry extract
作者：Liu, Yuxin;Shen, Chi;Wang, Xiaoyu;Tian, Rungang;Dong, Lijia;Han, Xueyuan;Xiao, Chaogeng;Lu, Wenjing;Luo, Zisheng;Han, Xueyuan;Sun, Guochang
关键词：Chinese rice wine; ethyl carbamate; raspberry extract; Saccharomyces cerevisiae; arginine metabolism
-
Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes
作者：Wang, Jing;Xiao, Chenwen;Liang, Shuang;Noman, Muhammad;Cai, Yingying;Zhang, Zhen;Zhu, Xueming;Chai, Rongyao;Qiu, Haiping;Hao, Zhongna;Wang, Yanli;Wang, Jiaoyu;Sun, Guochang;Lin, Fucheng;Bao, Guolian
关键词：
MoCDR1 ;TmCDR1 ; ABC transporters;Magnaporthe oryzae ;Trichophyton mentagrophytes -
Bacterial extracellular biomolecules-derived multimodal manganese nanoparticles control watermelon Fusarium wilt by dysregulating fusaric acid biosynthesis pathway and precise tuning of rhizosphere metabolome
作者：Noman, Muhammad;Islam, Mohammad Shafiqul;Wang, Jing;Cai, Yingying;Hao, Zhongna;Qiu, Haiping;Zhang, Zhen;Chai, Rongyao;Wang, Yanli;Wang, Jiaoyu;Ahmed, Temoor;Ahmed, Temoor;Islam, Mohammad Shafiqul;Li, Bin;Liang, Shuang;Ali, Hayssam M.
关键词：Fusaric acid; Fusarium wilt; Manganese nanoparticles; Rhizosphere metabolome; Watermelon
-
Antifungal profile and mechanism of bioinspired nanoscale magnesium against the agriculturally important pathogen Fusarium oxysporum f. sp. niveum
作者：Noman, Muhammad;Islam, Mohammad Shafiqul;Wang, Jing;Cai, Yingying;Hao, Zhongna;Zhang, Zhen;Wang, Yanli;Wang, Jiaoyu;Ahmed, Temoor;Islam, Mohammad Shafiqul;Li, Bin;Ahmed, Temoor;Ahmed, Temoor;Ahmad, Mudassar;Ali, Hayssam M.
关键词：
-
Utilizing zinc oxide nanoparticles as an environmentally safe biosystem to mitigate mycotoxicity and suppress Fusarium graminearium colonization in wheat
作者：Ibrahim, Ezzeldin;Hafeez, Rahila;Ogunyemi, Solabomi Olaitan;Abdallah, Yasmine;Li, Bin;Ibrahim, Ezzeldin;Xu, Lihui;Nasser, Raghda;Nasser, Raghda;Adel, Al-Shimaa Mohammed;Zhang, Zhen;Shou, Linfei;Wang, Daoze
关键词：ZnONPs; Antifungal mechanisms; DON reduction; Fusarium graminearum colonization; Plant protection
-
Modulation of rhizosphere microbial community and metabolites by bio-functionalized nanoscale silicon oxide alleviates cadmium-induced phytotoxicity in bayberry plants
作者：Ahmed, Temoor;Qi, Yetong;Yao, Yanlai;Qi, Xingjiang;Ahmed, Temoor;Guo, Junning;Li, Bin;Shou, Linfei;Noman, Muhammad;Masood, Hafiza Ayesha;Masood, Hafiza Ayesha;Rizwan, Muhammad;Ali, Md. Arshad;Ali, Hayssam M.;Yao, Yanlai
关键词：Antioxidants; Biogenic nanoparticles; Bacterial community; Heavy metals; Metabolomics; Silicon
