Phytophthora infestans RXLR effectors act in concert at diverse subcellular locations to enhance host colonization
文献类型： 外文期刊
作者： Wang, Shumei 1 ; McLellan, Hazel 1 ; Bukharova, Tatyana 1 ; He, Qin 1 ; Murphy, Fraser 1 ; Shi, Jiayang 3 ; Sun, Shaoh 1 ;
作者机构： 1.Univ Dundee, James Hutton Inst, Div Plant Sci, Dundee DD2 5DA, Scotland
2.James Hutton Inst, Cell & Mol Sci, Dundee DD2 5DA, Scotland
3.Huazhong Agr Univ, Minist Agr, Key Lab Potato Biol & Biotechnol, Wuhan 430070, Hubei, Peoples R China
4.Heilongjiang Bayi Agr Univ, Daqing 163319, Peoples R China
5.Heilongjiang Acad Agr Sci, Virus Free Seedling Res Inst, 368 Xuefu Rd, Harbin 150086, Heilongjiang, Peoples R China
6.Tech Univ Munich, Sch Life Sci Weihenstephan, Emil Ramann Str 2, D-85354 Freising Weihenstephan, Germany
7.Wageningen Univ, Wageningen UR Plant Breeding, POB 386, NL-6700 AJ Wageningen, Netherlands
关键词： Avirulence; biotrophy; effector-triggered susceptibility; pathogenicity; Phytophthora; RXLR effector; virulence
期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）
ISSN： 0022-0957
年卷期： 2019 年 70 卷 1 期
页码：
收录情况： SCI
摘要： Oomycetes such as the potato blight pathogen Phytophthora infestans deliver RXLR effectors into plant cells to manipulate host processes and promote disease. Knowledge of where they localize inside host cells is important in understanding their function. Fifty-two P infestans RXLR effectors (PiRXLRs) up-regulated during early stages of infection were expressed as fluorescent protein (FP) fusions inside cells of the model host Nicotiana benthamiana. FP-PiRXLR fusions were predominantly nucleo-cytoplasmic, nuclear, or plasma membrane-associated. Some also localized to the endoplasmic reticulum, mitochondria, peroxisomes, or microtubules, suggesting diverse sites of subcellular activity. Seven of the 25 PiRXLRs examined during infection accumulated at sites of haustorium penetration, probably due to co-localization with host target processes; Pi16663 (Avr1), for example, localized to Sec5-associated mobile bodies which showed perihaustorial accumulation. Forty-five FP-RXLR fusions enhanced pathogen leaf colonization when expressed in Nicotiana benthamiana, revealing that their presence was beneficial to infection. Co-expression of PiRXLRs that target and suppress different immune pathways resulted in an additive enhancement of colonization, indicating the potential to study effector combinations using transient expression assays. We provide a broad platform of high confidence P infestans effector candidates from which to investigate the mechanisms, singly and in combination, by which this pathogen causes disease.
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