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Detection of viral components in exosomes derived from NDV-infected DF-1 cells and their promoting ability in virus replication

文献类型： 外文期刊

作者： Xu, Xiaohong 1 ; Qian, Jing 2 ; Ding, Jiaxin 1 ; Li, Jindou 1 ; Nan, Fulong 1 ; Wang, Weiqi 1 ; Qin, Qi 1 ; Fei, Yidong 1 ;

作者机构： 1.Jilin Univ, Minist Educ, Key Lab Zoonosis Res, Lab Infect Dis,Coll Vet Med, Changchun 130062, Jilin, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Vet Bioprod Engn, Minist Agr, Inst Vet Med, Nanjing 210014, Jiangsu, Peoples R China

3.Linyi Univ, Coll Agr & Forestry Sci, Shuangling Rd, Linyi 276005, Shandong, Peoples R China

4.Jilin Agr Univ, Coll Anim Sci & Technol, Engn Res Ctr Jilin Prov Anim Probiot, Changchun 130118, Jilin, Peoples R China

关键词： Newcastle disease virus (NDV); Exosome; Protein A/G; Nucleoprotein (NP); Infection

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2019 年 128 卷

页码：

收录情况： SCI

摘要： Exosomes are micro messengers encapsulating RNA, DNA, and proteins for intercellular communication associated with various physiological and pathological reactions. Several viral infection processes have been reported to pertain to exosomal pathways. However, because of the difficulty in obtaining avian-sourced exosomes, avian virus-related exosomes are scarcely investigated. In this study, we developed a protein A/G-correlated method and successfully obtained the Newcastle disease virus-related exosome (NDV Ex). These exosomes promoted NDV propagation, proven by both GW4869-mediated deprivation and exosomal supplementation. Viral structural proteins NP and F were detected in the NDV Ex and further investigation indicated that the NP protein can be transferred to DF-1 cells through exosomes. The intracellular NP protein exhibited viral replication-promoting and cytokine-suppressing abilities. Therefore, NDV infection produces exosomes, which transfer viral NP protein and promote NDV infection, emphasizing the importance of exosomes in an NDV infection.

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