您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A magnetic nanoparticle based immunoassay for alternariol monomethyl ether using hydrogen peroxide-mediated fluorescence quenching of CdTe quantum dots

文献类型： 外文期刊

作者： Man, Yan 1 ; Jin, Xinxin 1 ; Fu, Hailong 1 ; Pan, Ligang 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China

2.Minist Agr, Risk Assessment Lab Agroprod Beijing, Beijing 100097, Peoples R China

3.Beijing Municipal Key Lab Agr Environm Monitoring, Beijing 100097, Peoples R China

关键词： Alternaria; Mycotoxin; Nanomaterial; Catalase; Sensitive detection; H2O2; Competitive assay; Food; Sample preparation

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN： 0026-3672

年卷期： 2019 年 186 卷 4 期

页码：

收录情况： SCI

摘要： The authors describe a fluorometric immunoassay for alternariol monomethyl ether (AME). It is making use of magnetic nanoparticles and quenching of the fluorescence of mercaptopropionic acid-capped CdTe quantum dots (MPA-CdTe QDs) by H2O2. Catalase (CAT) was labeled with AME as a competitive antigen to competitively bind to magnetic nanoparticles carrying monoclonal antibodies (mAbs) with free AME in samples. The effects of the concentration and pH value of buffer, the concentrations of H2O2 and CAT-AME, and the incubation time of H2O2 and MPA-CdTe QDs were optimized. Under optimal conditions and in combination with magnetic separation, the quenching of the fluorescence of the MPA-CdTe QDs (excitation at 310nm, emission at 599nm) can be used to quantify AME with a detection limit of 0.25pg mL( -1) and the linear range from 0.25 to 7.5pg mL( -1). The immunoassay also has a lower cross-reactivity to AME analogues. It was evaluated by analyzing fruit samples spiked with AME. The recoveries from spiked fruits ranged from 87.2% to 92.0%.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台