Covalent modification of beta-lactoglobulin by (-)-epigallocatechin-3-gallate results in a novel antioxidant molecule
文献类型： 外文期刊
作者： Tao, Fei 1 ; Xiao, Chaogeng 2 ; Chen, Wenwei 3 ; Zhang, Yongyong 3 ; Pan, Jiarong 3 ; Jia, Zhenbao 3 ;
作者机构： 1.China Jiliang Univ, Coll Standardizat, Hangzhou 310018, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China
3.China Jiliang Univ, Key Lab Marine Food Qual & Hazard Controlling Tec, Hangzhou 310018, Zhejiang, Peoples R China
关键词： beta-Lactoglobulin; (-)-Epigallocatechin-3-gallate; Modification; Antioxidant
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2019 年 126 卷
页码：
收录情况： SCI
摘要： beta-lactoglobulin (beta-lg), the predominant protein in bovine whey, was chemically modified by (-)-epigallocatechin-3-gallate (EGCG) to develop a biomacromolecule with antioxidant activity. The EGCG-modified beta-lg was characterized by SDS-PAGE, MALDI-TOF MS and intrinsic fluorescence spectroscopy. The antioxidant properties of EGCG-modified beta-lg was assessed using DPPH radical scavenging activity, iron chelating ability, and inhibition of Cu2+-induced LDL oxidation. SDS-PAGE and MALDI-TOF MS results indicated the dimerization of beta-lg after EGCG modification. Intrinsic fluorescence spectra suggested that EGCG modification caused an alteration in the conformational structure of beta-lg. The results demonstrated that EGCG-modified beta-lg possessed great antioxidant potential in terms of scavenging DPPH radical and chelating ferrous ion. Furthermore, the EGCG-modified beta-lg showed a protective effect against LDL peroxidation. The results indicate that EGCG-modified beta-lg could provide significant health benefits as an antioxidant. (C) 2019 Published by Elsevier B.V.
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