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Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis

文献类型： 外文期刊

作者： Congcong Wang 1 ; Liu, Hao 1 ; Yu, Sheng 3 ; Chen, Houbin 1 ; Hu, Fuchu 4 ; Zhan, Huiling 1 ; Pan, Xifen 1 ; Lao, Yuhua 1 ;

作者机构： 1.South China Agr Univ, Coll Hort, Guangdong Litchi Engn Res Ctr, Guangzhou 510642, Guangdong, Peoples R China

2.Guangdong Acad Forestry, Guangzhou 510520, Guangdong, Peoples R China

3.Chinese Univ Hong Kong, Sch Life Sci, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China

4.Hainan Acad Agr Sci, Key Lab Trop Fruit Trees, Haikou 571100, Hainan, Peoples R China

关键词： panicle leaf; senescence; transcriptome; litchi; low temperature

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN： 2073-4425

年卷期： 2019 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Warm winters and hot springs may promote panicle leaf growing and repress floral development. To identify genes potentially involved in litchi panicle leaf senescence, eight RNA-sequencing (RNA-Seq) libraries of the senescing panicle leaves under low temperature (LT) conditions and the developing panicle leaves under high temperature (HT) conditions were constructed. For each library, 4.78-8.99 x 10(6) clean reads were generated. Digital expression of the genes was compared between the senescing and developing panicle leaves. A total of 6477 upregulated differentially expressed genes (DEGs) (from developing leaves to senescing leaves), and 6318 downregulated DEGs were identified, 158 abscisic acid (ABA)-, 68 ethylene-, 107 indole-3-acetic acid (IAA)-, 27 gibberellic acid (GA)-, 68 cytokinin (CTK)-, 37 salicylic acid (SA)-, and 23 brassinolide (BR)-related DEGs. Confirmation of the RNA-Seq data by quantitative real-time PCR (qRT-PCR) analysis suggested that expression trends of the 10 candidate genes using qRT-PCR were similar to those revealed by RNA-Seq, and a significantly positive correlation between the obtained data from qRT-PCR and RNA-Seq were found, indicating the reliability of our RNA-Seq data. The present studies provided potential genes for the future molecular breeding of new cultivars that can induce panicle leaf senescence and reduce floral abortion under warm climates.

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