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Overexpression of a soybean 4-coumaric acid: coenzyme A ligase (GmPI4L) enhances resistance to Phytophthora sojae in soybean

文献类型： 外文期刊

作者： Chen, Xi 1 ; Fang, Xin 1 ; Zhang, Youyi 1 ; Wang, Xin 1 ; Zhang, Chuanzhong 1 ; Yan, Xiaofei 1 ; Zhao, Yuanling 1 ; Wu, J 1 ;

作者机构： 1.Northeast Agr Univ, Chinese Educ Minist, Key Lab Soybean Biol, Soybean Res Inst, Harbin, Heilongjiang, Peoples R China

2.Heilongjiang Acad Land Reclamat Sci, Harbin, Heilongjiang, Peoples R China

3.Heilongjiang Acad Agr Sci, Biotechnol Res Inst, Harbin, Heilongjiang, Peoples R China

4.Heilongjiang Acad Agr Sci, Key Lab Soybean Cultivat, Minist Agr PR China, Soybean Res Inst, Harbin, Heilongjiang, Peoples R China

关键词： GmPI4L; pathogenic change; Phytophthora sojae; soybean

期刊名称：FUNCTIONAL PLANT BIOLOGY （ 影响因子：3.101； 五年影响因子：3.248 ）

ISSN： 1445-4408

年卷期： 2019 年 46 卷 4 期

页码：

收录情况： SCI

摘要： Phytophthora root and stem rot of soybean (Glycine max (L.) Merr.) caused by Phytophthora sojae is a destructive disease worldwide. The enzyme 4-coumarate: CoA ligase (4CL) has been extensively studied with regard to plant responses to pathogens. However, the molecular mechanism of the response of soybean 4CL to P. sojae remains unclear. In a previous study, a highly upregulated 4CL homologue was characterised through suppressive subtractive hybridisation library and cDNA microarrays, in the resistant soybean cultivar Suinong 10' after infection with P. sojae race 1. Here, we isolated the full-length EST, and designated as GmPI4L (P. sojae-inducible 4CL gene) in this study, which is a novel member of the soybean 4CL gene family. GmPI4L has 34-43% over all amino acid sequence identity with other plant 4CLs. Overexpression of GmPI4L enhances resistance to P. sojae in transgenic soybean plants. The GmPI4L is located in the cell membrane when transiently expressed in Arabidopsis protoplasts. Further analyses showed that the contents of daidzein, genistein, and the relative content of glyceollins are significantly increased in overexpression GmPI4L soybeans. Taken together, these results suggested that GmPI4L plays an important role in response to P. sojae infection, possibly by enhancing the content of glyceollins, daidzein, and genistein in soybean.

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