Development of a QuEChERS-Based UHPLC-MS/MS Method for Simultaneous Determination of Six Alternaria Toxins in Grapes
文献类型： 外文期刊
作者： Guo, Wenbo 1 ; Fan, Kai 1 ; Nie, Dongxia 1 ; Meng, Jiajia 1 ; Huang, Qingwen 1 ; Yang, Junhua 1 ; Shen, Yuanyuan 1 ; Tang 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China
2.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China
3.Sciensano, Organ Contaminants & Addit, B-3080 Tervuren, Belgium
关键词： Alternaria toxins; grape; modified QuEChERS; UHPLC-MS; MS
期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）
ISSN： 2072-6651
年卷期： 2019 年 11 卷 2 期
页码：
收录情况： SCI
摘要： A simple and reliable analytical method for the simultaneous determination of alternariol (AOH), altenuene (ALT), tentoxin (TEN), altenusin (ALS), tenuazonic acid (TeA), and alternariol monomethyl ether (AME) in grapes was developed by ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure with the extraction by acetonitrile and purification by sodium chloride (0.5 g) and anhydrous magnesium sulfate (0.5 g) was established to recover the six Alternaria toxins. After validation by determining the linearity (R-2 > 0.99), recovery (77.8-101.6%), sensitivity (limit of detection in the range of 0.03-0.21 g kg(-1), and limit of quantification in the range of 0.09-0.48 g kg(-1)), and precision (relative standard deviation (RSD) 12.9%), the analytical method was successfully applied to reveal the contamination state of Alternaria toxins in grapes. Among 56 grape samples, 40 (incidence of 71.4%) were contaminated with Alternaria toxins. TEN was the most frequently found mycotoxin (37.5%), with a concentration range of 0.10-1.64 g kg(-1), followed by TeA (28.6%) and AOH (26.8%). ALT (10.7%), AME (3.6%), and ALS (5.4%) were also detected in some samples. To the best of our knowledge, this is the first report about the Alternaria toxins contamination in grapes in China.
- 相关文献
作者其他论文 更多>>
-
Preparation and characterization of the aflatoxin B1 purity certified reference material (GBW (E) 100599)
作者：Guo, Wenbo;Li, Zenghe;Guo, Wenbo;Meng, Jiajia;Wang, Xinyi;Li, Jianchi;Niu, Xueke;Zhao, Zhihui;Han, Zheng;Guo, Wenbo;Niu, Xueke
关键词：aflatoxin B1; Mass balance method; Quantitative nuclear magnetic resonance; Uncertaintity; Purity certified reference material
-
Assessing Seasonal Effects on Identification of Cultivation Methods of Short-Growth Cycle Brassica chinensis L. Using IRMS and NIRS
作者：Liu, Xing;Fan, Kai;Lu, Yangyang;Zhao, Hong;Rao, Qinxiong;Geng, Hao;Chen, Yijiao;Song, Weiguo;Liu, Xing;Fan, Kai;Lu, Yangyang;Zhao, Hong;Rao, Qinxiong;Geng, Hao;Chen, Yijiao;Song, Weiguo;Rogers, Karyne Maree;Rogers, Karyne Maree
关键词：Brassica chinensis L.; seasonal effect; stable isotope; NIRS; cultivation method; PLS-DA
-
The protective effects of Lactobacillus SNK-6 on growth, organ health, and intestinal function in geese exposed to low concentration Aflatoxin B1
作者：Li, Guangquan;Wang, Huiying;Liu, Yi;Wang, Xianze;Yan, Huaxiang;He, Daqian;Yang, Junhua;Qiu, Zhi
关键词：goose; growth performance; liver; intestinal; cecum microbiota
-
Lactobacillus salivarius Ameliorates AFB1-induced hepatotoxicity via PINK1/Parkin-mediated mitophagy in Geese
作者：Qiu, Zhi;Wang, Xichun;Wang, Huiying;Li, Guangquan;Liu, Yi;He, Daqian;Qiu, Zhi;Wang, Xianze;Yang, Junhua
关键词：Lactobacillus salivarius; Aflatoxin B1; Hepatotoxicity; Mitochondrial autophagy; PINK1 /Parkin pathway; Goose
-
Co-contamination and interactions of multiple mycotoxins and heavy metals in rice, maize, soybeans, and wheat flour marketed in Shanghai City
作者：Zhu, Zuoyin;Guo, Wenbo;Cheng, Haisheng;Zhao, Hanke;Wang, Jie;Yang, Junhua;Zhu, Zuoyin;Cheng, Haisheng;Wang, Jie;Zhou, Xinli;Abdallah, Mohamed F.;Abdallah, Mohamed F.;Lei, Hulong;Tu, Weilong;Wang, Hongyang
关键词：Grains and grain products; Mycotoxins; Heavy metals; Combined contamination; Interactivity analysis
-
Screening and dietary exposure assessment of T-2 toxin and its modified forms in commercial cereals and cereal-based products in Shanghai
作者：Guo, Wenbo;Feng, Disen;Yang, Xianli;Zhao, Zhihui;Yang, Junhua;Guo, Wenbo
关键词：T-2 toxin; Modified mycotoxins; Trichothecenes; UPLC-MS/MS; Cereals and cereal-based products; Dietary exposure assessment
-
Perspective Accelerated photodegradation of T-2 toxin over magnetic recyclable ZnO/ CaFe 2 O 4 nanocomposite with a p-n based Z-scheme heterojunction architecture
作者：Huang, Qingwen;Lou, Xiuping;Nie, Dongxia;Zhao, Zhihui;Fan, Kai;Guo, Wenbo;Meng, Jiajia;Han, Zheng;Lou, Xiuping;Han, Zheng;Liu, Zheyuan
关键词：T-2 toxin; Photocatalytic degradation; Z-scheme; p-n heterojunction
