Highly Efficient and Enzyme-Recoverable Method for Enzymatic Concentrating Omega-3 Fatty Acids Generated by Hydrolysis of Fish Oil in a Substrate-Constituted Three-Liquid-Phase System
文献类型： 外文期刊
作者： Li, Zhigang 1 ; Chen, Hua 1 ; Su, Jinfen 1 ; Wang, Weifei 3 ; Chen, Huayong 1 ; Yang, Bo 1 ; Wang, Yonghua 4 ;
作者机构： 1.South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Guangdong, Peoples R China
2.South China Univ Technol, Guangdong Prov Key Lab Fermentat & Enzyme Engn, Guangzhou 510006, Guangdong, Peoples R China
3.Guangdong Acad Agr Sci, Minist Agr, Sericultural & Agrifood Res Inst, Guangdong Key Lab Agr Prod Proc,Key Lab Funct Foo, Guangzhou 510610, Guangdong, Peoples R China
4.South China Univ Technol, Sch Light Ind & Food Sci, Guangzhou 510641, Guangdong, Peoples R China
关键词： three-liquid-phase system; n-3 polyunsaturated fatty acids; lipase; fish oil; hydrolysis
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2019 年 67 卷 9 期
页码：
收录情况： SCI
摘要： A novel three-liquid-phase system which contained fish oil as the nonpolar phase was developed for the lipase-based hydrolysis of fish oil and subsequent enrichment of the omega-3 polyunsaturated fatty acids (n-3 PUFA) in the glyceride fraction of the fish oil. In comparison with the traditional oil/water system, the enrichment factor of n-3 PUFA in this system was increased by 363.4% as a result of a higher dispersity, higher selectivity of the lipase for the other fatty acids except for n-3PUFA, and relief of product inhibition. The content of n-3 PUFA in the glyceride fraction could be concentrated to 67.97% by repeated hydrolysis after removing the free fatty acids. Furthermore, the lipase could be reused for at least eight rounds. This method would be an ideal approach for enriching n-3 PUFA because it is cost-effective, low in toxicity, and easily scaled up.
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