您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and characterization of FRUITFULL-like genes from Platanus acerifolia, a basal eudicot tree

文献类型： 外文期刊

作者： Zhang, Sisi 1 ; Lu, Shunjiao 1 ; Yi, Shuangshuang 1 ; Han, Hongji 1 ; Zhou, Qin 1 ; Cai, Fangfang 1 ; Bao, Manzhu 1 ; Liu 1 ;

作者机构： 1.Huazhong Agr Univ, Key Lab Urban Agr Cent China, Coll Hort & Forestry Sci, Key Lab Hort Plant Biol,Minist Educ,Minist Agr, Shizishan St 1, Wuhan 430070, Peoples R China

2.Wuhan Inst Landscape Architecture, Peace Ave 1240, Wuhan 430081, Hubei, Peoples R China

3.Guangzhou Inst Forestry & Landscape Architecture, Guangzhou 510405, Guangdong, Peoples R China

4.CATAS, Trop Crops Genet Resources Inst, Key Lab Crop Gene Resources & Germplasm Enhanceme, Minist Agr, Danzhou 571737, Peoples R China

5.Nanyang Normal Univ, Coll Agr Engn, Nanyang 473061, Peoples R China

关键词： Basal eudicot; Dormancy; FRUITFULL; Floral development; London plane; MADS-box; Protein-protein interaction

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2019 年 280 卷

页码：

收录情况： SCI

摘要： The function of euAP1 and euFUL in AP1/FUL lineage have been well characterized in core eudicots, and they play common and distinct roles in plant development. However, the evolution and function of FUL-like genes is poorly understood in basal eudicots. In this study, we identified three FUL-like genes PlacFL1/2/3 from London plane (Platanus acerifolia). Sequence alignment and phylogenetic analysis indicated that PlacFL1/2/3 are AP1/FUL orthologs and encoded proteins containing FUL motif and paleoAP1 motif. Quantitative real-time PCR (qRT-PCR) analysis showed that PlacFL1/2/3 were expressed in both vegetative and reproductive tissues, but with distinct spatiotemporal patterns. In contrast to PlacFL1 and PIacFL3, PlacFL2 exhibited higher expression levels and broader expression regions, and that the expression of PlacFL2 gene showed a decreasing and increasing tendency in subpetiolar buds during dormancy induction and breaking, respectively. Overexpression of PlacFLs in Arabidopsis and PlacFL3 in tobacco resulted in early flowering, as well as early termination of inflorescence meristems for transgenic Arabidopsis plants. The expression changes of flowering time and flower meristem identity genes in transgenic Arabidopsis lines with different PlacFLs suggested that PlacFL2 and PIacFL3 may regulate different downstream genes to perform divergent functions. Yeast two-hybrid analysis indicated that PlacFLs interacted strongly with PlacSEP proteins, and PlacFL3 instead of PlacFL1 and PlacFL2 could also form a homodimer and interact with D-class proteins. Our results suggest that PlacFLs may play conserved functions in regulating flowering and flower development, and PlacFL2 might also be involved in dormancy regulation. The research helps us to understand the functional evolution of FUL-like genes in basal eudicots, especially in perennial woody species.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台