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MeGLYI-13, a Glyoxalase I Gene in Cassava, Enhances the Tolerance of Yeast and Arabidopsis to Zinc and Copper Stresses

文献类型： 外文期刊

作者： Li, Ruimei 1 ; Tang, Fenlian 1 ; Che, Yannian 1 ; Fernie, Alisdair R. 4 ; Zhou, Qin 1 ; Ding, Zhongping 1 ; Yao, Yuan 1 ; Liu, Jiao 1 ; Wang, Yajie 1 ; Hu, Xinwen 3 ; Guo, Jianchun 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Peoples R China

2.Hainan Inst Trop Agr Resources, Key Lab Biol & Genet Resources Trop Crops Hainan, Haikou 571101, Peoples R China

3.Hainan Univ, Coll Trop Crops, Haikou 570228, Peoples R China

4.Max Planck Inst Mol Plant Physiol, Root Biol & Symbiosis, Muhlenberg 1, D-14476 Potsdam, Germany

关键词： glyoxalase; cassava; heavy metal; zinc; copper; overexpression; yeast; Arabidopsis; subcellular localization

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 12 卷 19 期

页码：

收录情况： SCI

摘要： Although zinc and copper are the two essential nutrients necessary for plant growth, their excessive accumulation in soil not only causes environmental pollution but also seriously threatens human health and inhibits plant growth. The breeding of plants with novel zinc or copper toxicity tolerance capacities represents one strategy to address this problem. Glyoxalase I (GLYI) family genes have previously been suggested to be involved in the resistance to a wide range of abiotic stresses, including those invoked by heavy metals. Here, a MeGLYI-13 gene cloned from a cassava SC8 cultivar was characterized with regard to its potential ability in resistance to zinc or copper stresses. Sequence alignment indicated that MeGLYI-13 exhibits sequence differences between genotypes. Transient expression analysis revealed the nuclear localization of MeGLYI-13. A nuclear localization signal (NLS) was found in its C-terminal region. There are 12 Zn2+ binding sites and 14 Cu2+ binding sites predicted by the MIB tool, of which six binding sites were shared by Zn2+ and Cu2+. The overexpression of MeGLYI-13 enhanced both the zinc and copper toxicity tolerances of transformed yeast cells and Arabidopsis seedlings. Taken together, our study shows the ability of the MeGLYI-13 gene to resist zinc and copper toxicity, which provides genetic resources for the future breeding of plants resistant to zinc and copper and potentially other heavy metals.

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