Promoter of Cassava MeAHL31 Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic Arabidopsis
文献类型： 外文期刊
作者： Wang, Xiao-Tong 1 ; Tang, Xiang-Ning 1 ; Zhang, Ya-Wen 1 ; Guo, Yu-Qiang 1 ; Yao, Yuan 2 ; Li, Rui-Mei 2 ; Wang, Ya-Jie 2 ; Liu, Jiao 2 ; Guo, Jian-Chun 2 ;
作者机构： 1.Hainan Univ, Sch Life & Hlth Sci, Natl Key Lab Trop Crop Breeding, Haikou 570228, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Natl Key Lab Trop Crop Breeding, Sanya Res Inst, Haikou 571101, Peoples R China
关键词： cassava; AT-hook protein; promoter; GUS; transgenic Arabidopsis; abiotic stresses; hormone signals; MeAHL31
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.6 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 14 期
页码：
收录情况： SCI
摘要： The AT-hook motif nuclear-localized (AHL) family is pivotal for the abiotic stress response in plants. However, the function of the cassava AHL genes has not been elucidated. Promoters, as important regulatory elements of gene expression, play a crucial role in stress resistance. In this study, the promoter of the cassava MeAHL31 gene was cloned. The MeAHL31 protein was localized to the cytoplasm and the nucleus. qRT-PCR analysis revealed that the MeAHL31 gene was expressed in almost all tissues tested, and the expression in tuber roots was 321.3 times higher than that in petioles. Promoter analysis showed that the MeAHL31 promoter contains drought, methyl jasmonate (MeJA), abscisic acid (ABA), and gibberellin (GA) cis-acting elements. Expression analysis indicated that the MeAHL31 gene is dramatically affected by treatments with salt, drought, MeJA, ABA, and GA3. Histochemical staining in the proMeAHL31-GUS transgenic Arabidopsis corroborated that the GUS staining was found in most tissues and organs, excluding seeds. Beta-glucuronidase (GUS) activity assays showed that the activities in the proMeAHL31-GUS transgenic Arabidopsis were enhanced by different concentrations of NaCl, mannitol (for simulating drought), and MeJA treatments. The integrated findings suggest that the MeAHL31 promoter responds to the abiotic stresses of salt and drought, and its activity is regulated by the MeJA hormone signal.
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