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Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava

文献类型： 外文期刊

作者： Lu, Xiao-Hua 1 ; Wang, Ya-Jie 2 ; Zhen, Xing-Hou 1 ; Yu, Hui 2 ; Pan, Mu 1 ; Fu, Dong-Qing 3 ; Li, Rui-Mei 2 ; Liu, Jiao 2 ; Luo, Hai-Yan 4 ; Hu, Xin-Wen 1 ; Yao, Yuan 2 ; Guo, Jian-Chun 2 ;

作者机构： 1.Hainan Univ, Sch Life & Hlth Sci, Natl Key Lab Trop Crop Breeding, Haikou 571101, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Natl Key Lab Trop Crop Breeding, Sanya Res Inst, Haikou 571101, Peoples R China

3.Hainan Univ, Sch Trop Agr & Forestry, Haikou 570228, Peoples R China

4.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Peoples R China

关键词： cassava; starch synthase; MeSSIII-1; promoter; hormones; starch synthesis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 9 期

页码：

收录情况： SCI

摘要： Soluble starch synthases (SSs) play important roles in the synthesis of cassava starch. However, the expression characteristics of the cassava SSs genes have not been elucidated. In this study, the MeSSIII-1 gene and its promoter, from SC8 cassava cultivars, were respectively isolated by PCR amplification. MeSSIII-1 protein was localized to the chloroplasts. qRT-PCR analysis revealed that the MeSSIII-1 gene was expressed in almost all tissues tested, and the expression in mature leaves was 18.9 times more than that in tuber roots. MeSSIII-1 expression was induced by methyljasmonate (MeJA), abscisic acid (ABA), and ethylene (ET) hormones in cassava. MeSSIII-1 expression patterns were further confirmed in proMeSSIII-1 transgenic cassava. The promoter deletion analysis showed that the -264 bp to -1 bp MeSSIII-1 promoter has basal activity. The range from -1228 bp to -987 bp and -488 bp to -264 bp significantly enhance promoter activity. The regions from -987 bp to -747 bp and -747 bp to -488 bp have repressive activity. These findings will provide an important reference for research on the potential function and transcriptional regulation mechanisms of the MeSSIII-1 gene and for further in-depth exploration of the regulatory network of its internal functional elements.

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