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An Anthocyanin-Based Visual Reporter System for Genetic Transformation and Genome Editing in Cassava

文献类型： 外文期刊

作者： Zhen, Xing-Hou 1 ; Pan, Ran-Ran 2 ; Lu, Xiao-Hua 1 ; Ge, Yu-Jian 3 ; Li, Rui-Mei 2 ; Liu, Jiao 2 ; Wang, Ya-Jie 2 ; Yi, Ke-Xian 4 ; Li, Chun-Xia 1 ; Guo, Jian-Chun 1 ; Yao, Yuan 2 ; Geng, Meng-Ting 1 ;

作者机构： 1.Hainan Univ, Sch Life & Hlth Sci, Natl Key Lab Trop Crop Breeding, Haikou 570228, Peoples R China

2.Chinese Acad Trop Agr Sci, Sanya Res Inst, Inst Trop Biosci & Biotechnol, Natl Key Lab Trop Crop Breeding,Key Lab Biol & Gen, Haikou 571101, Peoples R China

3.Hainan Univ, Sch Trop Agr & Forestry, Haikou 570228, Peoples R China

4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Sanya Res Inst, Haikou 571101, Peoples R China

关键词： cassava anthocyanins; genetic transformation; genome editing; visual reporter

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 21 期

页码：

收录情况： SCI

摘要： Cassava (Manihot esculenta Crantz) is a staple crop in tropical and subtropical regions, valued for its high starch content in roots. Effective genetic transformation and genome editing of cassava require efficient screening methods for transgenic and edited plants. In this study, a visual selection marker system using an R2R3-MYB transcription factor anthocyanin 1 gene (HbAN1, LOC110667474) from a rubber tree (Hevea brasiliensis M & uuml;ll. Arg.) has been developed to facilitate the identification of transgenic cassava plants. Transgenic cassava lines expressing HbAN1 accumulated anthocyanins in their leaves, allowing for easy visual identification without the need for destructive assays or specialized equipment. Importantly, the accumulation of anthocyanins did not affect the regeneration or transformation efficiency of cassava. Additionally, the AR-CRISPR/Cas9-gRNA system with the HbAN1 gene as a marker produced MeCDD4 gene-edited cassava mutants with purple leaves, demonstrating successful editing. This anthocyanin-based visual reporter (AR) system will provide an effective tool for genetic transformation and genome editing in cassava.

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