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The Cotton Apoplastic Protein CRR1 Stabilizes Chitinase 28 to Facilitate Defense against the Fungal Pathogen Verticillium dahliae

文献类型： 外文期刊

作者： Han, Li-Bo 1 ; Li, Yuan-Bao 1 ; Wang, Fu-Xin 1 ; Wang, Wen-Yan 1 ; Liu, Jun 1 ; Wu, Jia-He 1 ; Zhong, Nai-Qin 1 ; Wu, She 1 ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Crop Sci, Plant Immun Ctr,Key Lab, Haixia Inst Sci & Technol,Minist Educ Genet Breed, Fuzhou 350002, Fujian, Peoples R China

3.Shanxi Acad Agr Sci, Inst Cotton Res, Yuncheng 044000, Shanxi, Peoples R China

期刊名称：PLANT CELL （ 影响因子：11.277； 五年影响因子：12.061 ）

ISSN： 1040-4651

年卷期： 2019 年 31 卷 2 期

页码：

收录情况： SCI

摘要： The apoplast serves as the first battlefield between the plant hosts and invading microbes; therefore, work on plant-pathogen interactions has increasingly focused on apoplastic immunity. In this study, we identified three proteins in the apoplast of cotton (Gossypium sp) root cells during interaction of the plant with the fungal pathogen Verticillium dahliae. Among these proteins, cotton host cells secrete chitinase 28 (Chi28) and the Cys-rich repeat protein 1 (CRR1), while the pathogen releases the protease VdSSEP1. Biochemical analysis demonstrated that VdSSEP1 hydrolyzed Chi28, but CRR1 protected Chi28 from cleavage by Verticillium dahliae secretory Ser protease 1 (VdSSEP1). In accordance with the in vitro results, CRR1 interacted with Chi28 in yeast and plant cells and attenuated the observed decrease in Chi28 level that occurred in the apoplast of plant cells upon pathogen attack. Knockdown of CRR1 or Chi28 in cotton plants resulted in higher susceptibility to V. dahliae infection, and overexpression of CRR1 increased plant resistance to V. dahliae, the fungus Botrytis cinerea, and the oomycete Phytophthora parasitica var nicotianae. By contrast, knockout of VdSSEP1 in V. dahliae destroyed the pathogenicity of this fungus. Together, our results provide compelling evidence for a multilayered interplay of factors in cotton apoplastic immunity.

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