文献类型： 外文期刊

作者： Du, Haiping ¹ ; Li, Xiao ¹ ; Ning, Lihua ² ; Qin, Rui ¹ ; Du, Qing ¹ ; Wang, Qing ¹ ; Song, Haina ³ ; Huang, Fang ¹ ; Wang, ¹ ;

作者机构： 1.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Natl Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Inst Germplasm Resources & Biotechnol, Nanjing 210014, Jiangsu, Peoples R China

3.PingDingshan Univ, Coll Chem & Chem Engn, Lab Ecol Restorat Hilly Area, Pingdingshan 467000, Peoples R China

关键词： Wild soybean (Glycine soja Sieb; & Zucc; ); Resistance to insects; Common cutworm; RNA-Seq analysis; Phosphate transporter

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2019 年 20 卷

页码：

收录情况： SCI

摘要： BackgroundCommon cutworm (CCW) is highly responsible for destabilizing soybean productivity. Wild soybean is a resource used by breeders to discover elite defensive genes.ResultsThe transcriptomes of two wild accessions (W11 and W99) with different resistance to CCW were analyzed at early- and late-induction time points. After induction, the susceptible accession W11 differentially expressed 1268 and 508 genes at the early and late time points, respectively. Compared with W11, the resistant accession W99 differentially expressed 1270 genes at the early time point and many more genes (2308) at the late time point. In total, 3836 non-redundant genes were identified in both lines. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that the differentially expressed genes (DEGs) in W99 at the late time point were mostly associated with specific processes and pathways. Among the non-redundant genes, 146 genes were commonly up-regulated in the treatment condition compared with the control condition at the early- and late-induction time points in both accessions used in this experiment. Approximately 40% of the common DEGs were related to secondary metabolism, disease resistance, and signal transduction based on their putative function. Excluding the common DEGs, W99 expressed more unique DEGs than W11. Further analysis of the 3836 DEGs revealed that the induction of CCW not only up-regulated defense-related genes, including 37 jasmonic acid (JA)-related genes, 171 plant-pathogen-related genes, and 17 genes encoding protease inhibitors, but also down-regulated growth-related genes, including 35 photosynthesis-related genes, 48 nutrition metabolism genes, and 28 auxin metabolism genes. Therefore, representative defense-related and growth-related genes were chosen for binding site prediction via co-expression of transcription factors (TFs) and spatial expression pattern analyses. In total, 53 binding sites of 28 TFs were identified based on 3 defense-related genes and 3 growth-related genes. Phosphate transporter PT1, which is a representative growth-related gene, was transformed into soybean, and the transgenic soybean plants were susceptible to CCW.ConclusionsIn summary, we described transcriptome reprograming after herbivore induction in wild soybean, identified the susceptibility of growth-related genes, and provided new resources for the breeding of herbivore-resistant cultivated soybeans.