文献类型： 外文期刊

作者： Wang, Qian ¹ ; Guan, Chao ² ; Wang, Pei ³ ; Ma, Qing ² ; Bao, Ai-Ke ² ; Zhang, Jin-Lin ² ; Wang, Suo-Min ² ;

作者机构： 1.Guizhou Acad Agr Sci, Guizhou Inst Prataculture, Guiyang 550006, Guizhou, Peoples R China

2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China

3.Southwest Minzu Univ, Inst Qinghai Tibetan Plateau, Chengdu 610041, Sichuan, Peoples R China

关键词： Arabidopsis thaliana; athkt1; 1; atsos1; Na+; K+; salt stress

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2019 年 20 卷 5 期

页码：

收录情况： SCI

摘要： HKT1 and SOS1 are two key Na+ transporters that modulate salt tolerance in plants. Although much is known about the respective functions of HKT1 and SOS1 under salt conditions, few studies have examined the effects of HKT1 and SOS1 mutations on the expression of other important Na+ and K+ transporter genes. This study investigated the physiological parameters and expression profiles of AtHKT1;1, AtSOS1, AtHAK5, AtAKT1, AtSKOR, AtNHX1, and AtAVP1 in wild-type (WT) and athkt1;1 and atsos1 mutants of Arabidopsis thaliana under 25 mM NaCl. We found that AtSOS1 mutation induced a significant decrease in transcripts of AtHKT1;1 (by 56-62% at 6-24 h), AtSKOR (by 36-78% at 6-24 h), and AtAKT1 (by 31-53% at 6-24 h) in the roots compared with WT. This led to an increase in Na+ accumulation in the roots, a decrease in K+ uptake and transportation, and finally resulted in suppression of plant growth. AtHKT1;1 loss induced a 39-76% (6-24 h) decrease and a 27-32% (6-24 h) increase in transcripts of AtSKOR and AtHAK5, respectively, in the roots compared with WT. At the same time, 25 mM NaCl decreased the net selective transport capacity for K+ over Na+ by 92% in the athkt1;1 roots compared with the WT roots. Consequently, Na+ was loaded into the xylem and delivered to the shoots, whereas K+ transport was restricted. The results indicate that AtHKT1;1 and AtSOS1 not only mediate Na+ transport but also control ion uptake and the spatial distribution of Na+ and K+ by cooperatively regulating the expression levels of relevant Na+ and K+ transporter genes, ultimately regulating plant growth under salt stress.