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Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum

文献类型： 外文期刊

作者： Cao, Dong 1 ; Fan, Jiequn 3 ; Xi, Xingyuan 1 ; Zong, Yuan 2 ; Wang, Dongxia 2 ; Zhang, Huaigang 1 ; Liu, Baolong 1 ;

作者机构： 1.Qinghai Prov Key Lab Crop Mol Breeding, Xining 810008, Qinghai, Peoples R China

2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 800010, Qinghai, Peoples R China

3.Shanghai Acad Agr Sci, Shanghai 201403, Peoples R China

关键词： T. monococcum; coleoptile; anthocyanin biosynthesis; MYB transcription factor

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2019 年 24 卷 5 期

页码：

收录情况： SCI

摘要： Red coleoptiles can help crops to cope with adversity and the key genes that are responsible for this trait have previously been isolated from Triticum aestivum, Triticum urartu, and Aegilops tauschii. This report describes the use of transcriptome analysis to determine the candidate gene that controls the trait for white coleoptiles in T. monococcum by screening three cultivars with white coleoptiles and two with red coleoptiles. Fifteen structural genes and two transcription factors that are involved in anthocyanin biosynthesis were identified from the assembled UniGene database through BLAST analysis and their transcript levels were then compared in white and red coleoptiles. The majority of the structural genes reflected lower transcript levels in the white than in the red coleoptiles, which implied that transcription factors related to anthocyanin biosynthesis could be candidate genes. The transcript levels of MYC transcription factor TmMYC-A1 were not significantly different between the white and red coleoptiles and all of the TmMYC-A1s contained complete functional domains. The deduced amino acid sequence of the MYB transcription factor TmMYB-A1 in red coleoptiles was homologous to TuMYB-A1, TaMYB-A1, TaMYB-B1, and TaMYB-D1, which control coleoptile color in corresponding species and contained the complete R2R3 MYB domain and the transactivation domain. TmMYB-a1 lost its two functional domains in white coleoptiles due to a single nucleotide deletion that caused premature termination at 13 bp after the initiation codon. Therefore, TmMYB-A1 is likely to be the candidate gene for the control of the red coleoptile trait, and its loss-of-function mutation leads to the white phenotype in T. monococcum.

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