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The cassava (Manihot-esculenta Crantz)'s nitrate transporter NPF4.5, expressed in seedling roots, involved in nitrate flux and osmotic stress

文献类型： 外文期刊

作者： Zou, Liangping 1 ; Qi, Dengfeng 1 ; Li, Shuxia 1 ; Zhai, Min 3 ; Li, Zhuang 3 ; Guo, Xin 4 ; Ruan, Mengbin 1 ; Yu, Xiaoling 1 ; Zhao, Pingjuan 1 ; Li, Wenbin 1 ; Zhang, Peng 5 ; Ma, Qiuxiang 5 ; Peng, Ming 1 ; Liao, Wenbin 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Peoples R China

2.Hainan Inst Trop Agr Resources, China Key Lab Biol & Genet Resources Trop Crops Ha, Haikou 571101, Peoples R China

3.Sichuan Agr Univ, Coll Agron, Chengdu 611130, Sichuan, Peoples R China

4.Coll Plant Sci & Technol HuaZhongAgricultural Univ, Wuhan 430070, Hubei, Peoples R China

5.Chinese Acad Sci, Inst Plant Physiol & Ecol, CAS Ctr Excellence & Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

6.Hainan Inst Trop Agr Resources, Key Lab Biol & Genet Resources Trop Crops Hainan P, Haikou 571101, Peoples R China

关键词： MeNPF4; 5; Nitrate flux; Osmotic stress; Cassava

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.437； 五年影响因子：5.731 ）

ISSN： 0981-9428

年卷期： 2023 年 194 卷

页码：

收录情况： SCI

摘要： AtNPF4.5/AIT2, which was predicted to be a low-affinity transporter capable for nitrate uptake, was screened by ABA receptor complex in Arabidopsis ten years ago. However, the molecular and biochemical characterizations of AtNPF4.5 in plants remained largely unclear. In this study, the function of a plasma-membrane-localized and root-specifically-expressed gene MeNPF4.5 (Manihot-esculenta NITRATE TRANSPORTER 1 PTR FAMILY4.5), an ortholog of the Arabidopsis thaliana NPF4.5, was investigated in cassava roots as a nitrate efflux transporter on low nitrate medium and an influx transporter following exposure to high concentration of external nitrates. Moreover, RNA interference (RNAi) of MeNPF4.5 reduced the nitrate efflux capacity but the overexpressing cassava seedlings increased the ability of efflux from the elongation to the mature zone of root under low nitrate treatments. Besides, MeNPF4.5-RNAi expression reduced the nitrate influx capacity but enhanced nitrate ab-sorption in parts of overexpressing plants from the meristem, elongation to mature zone of roots under high nitrate conditions. Furthermore, MeNPF4.5-RNAi seedlings survived owing to roots that could grow normally, but the MeNPF4.5-over-expressors showed adverse growth under 7% PEG6000 stress, suggesting that MeNPF4.5 negatively regulated the osmotic stress and was involved in nitrate flux through cassava seedlings.

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