Identification of the cassava NADP-ME gene family and its response and regulation in photosynthesis
文献类型： 外文期刊
作者： Li, Haozheng 1 ; Xiao, Jin 1 ; Chen, Jiahui 1 ; Shen, Xu 1 ; Luo, Jia 1 ; Guo, Fengguang 1 ; Wang, Shangfei 2 ; Xu, Liangye 1 ; Guo, Xin 1 ; Wang, Shujuan 1 ; Wang, Haiyan 3 ; Wang, Wenquan 1 ;
作者机构： 1.Hainan Univ, Sch Trop Agr & Forestry, Danzhou, Peoples R China
2.Guangxi Univ, Coll Agr, Nanning, Peoples R China
3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Natl Key Lab Trop Crop Breeding, Sanya Res Inst,Key Lab Biol & Genet Resources Trop, Haikou, Peoples R China
关键词： C-3-C-4 intermediate photosynthesis; NADP-ME; cassava; C-4 evolution; coexpression network
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）
ISSN： 1664-462X
年卷期： 2025 年 16 卷
页码：
收录情况： SCI
摘要： NADP-malic enzyme (NADP-ME) is a crucial enzyme in C-4 photosynthesis, responsible for the decarboxylation of malate in bundle sheath cells, enhancing the photosynthetic efficiency of C-4 plants. Cultivated cassava exhibits high photosynthetic efficiency and biomass, and previous studies classify it as a C-3-C-4 intermediate type. The biomass of cassava correlates positively with photosynthetic rate, and the promoter region of MeNADP-ME3 contains insertion selected in cultivars different from wild ancestors. Four MeNADP-ME genes were identified in the cultivated cassava variety AM560, with promoter regions enriched in light-responsive elements. Phylogenetic and conserved domain analyses revealed that all subtypes are plastidic dicotyledonous types, closely related to AtNADP-ME4, with unique N-terminal domains in MeNADP-ME2 and MeNADP-ME3 specific to cassava, suggesting new functional roles. Subcellular localization showed predominant chloroplast localization, with greater involvement in leaf physiological processes in the cultivated variety SC205. These findings suggest that the NADP-ME family in cultivated cassava has been evolutionarily selected for photosynthesis. Further investigation revealed that MeNADP-ME3 is highly expressed in leaves and regulated by light intensity. Co-expression network analysis of shade-treated transcriptomes and transcription factor-promoter predictions showed that Indel sites in the MeNADP-ME3 promoter are bound by MeYABBY1, forming a regulatory network with other photosynthesis-related genes. This suggests that MeNADP-ME3 plays a role in C-3-C-4 intermediate photosynthesis during the evolution from wild to cultivated cassava, with other family genes also evolving towards photosynthetic functions. Our study lays the foundation for future functional research on the MeNADP-ME family and provides insights into the mechanisms underlying the high photosynthetic efficiency of cultivated cassava.
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