Beyond nitrate transport: AtNRT2.4 responds to local and systemic nitrogen signaling in Arabidopsis
文献类型： 外文期刊
作者： Zhang, Huaifang 1 ; Huang, Jinxia 2 ; Li, Yanmei 1 ; Zhao, Jinling 1 ; Mai, Weitao 1 ; Khan, Luqman 1 ; Zhang, Meng 3 ; Wang, Wenquan 1 ; Zeng, Changying 1 ; Chen, Xin 1 ;
作者机构： 1.Hainan Univ, Sanya Inst Breeding & Multiplicat, Sch Trop Agr & Forestry, Natl Key Lab Trop Crop Breeding, Sanya, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Sanya Res Inst, Haikou, Peoples R China
3.Liaoning Acad Agr Sci, Shenyang, Peoples R China
关键词： Nitrate deficiency; Local N-deficiency signals; Systemic nitrate signals; Root architecture; Anthocyanin accumulation
期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）
ISSN： 1471-2229
年卷期： 2025 年 25 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundPlants have evolved the ability to detect nitrogen fluctuations to optimize their nitrogen acquisition. However, the mechanisms of nitrogen perception and signaling still need to be well characterized.ResultsThrough split-root experiments, this study demonstrated that nitrate transporter 2.4 (AtNRT2.4) can respond to both local and systemic nitrate signals, modulating the transcription of genes such as AtANR1 and AtCIPK23, thereby altering root architecture. Beyond merely detecting the fluctuations of environmental nitrate concentrations, AtNRT2.4 was actively engaged in the dual-affinity transition of AtNRT1.1 and suppressed the expression of AtNLP7, which is crucial for responding to intracellular nitrate signals. Notably, AtNRT2.4 did not participate in the CEP-mediated systemic nitrogen stress signaling pathway and also did not require AtNRT3.1 as a chaperone protein. The knockout of AtNRT2.4 did not affect the growth of Arabidopsis thaliana under low nitrate conditions. However, its overexpression significantly enhanced biomass accumulation and seed yield under normal nitrate concentrations. Furthermore, under nitrate deficiency stress, AtNRT2.4 induced the expression of key genes involved in anthocyanin synthesis and accumulation, thereby promoting anthocyanin accumulation in leaves.ConclusionsIn summary, AtNRT2.4 plays a crucial role in local and systemic nitrate signals sensing, adjustment of root architecture, and anthocyanin accumulation, providing new insights into how plants respond to nitrogen deprivation.
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