Endoplasmic reticulum remodeling induced by Wheat yellow mosaic virus infection studied by transmission electron microscopy
文献类型： 外文期刊
作者： Xie, Li 1 ; Song, Xi-jiao 2 ; Liao, Zhen-feng 2 ; Wu, Bin 3 ; Yang, Jian 2 ; Zhang, Hengmu 2 ; Hong, Jian 1 ;
作者机构： 1.Zhejiang Univ, Anal Ctr Agrobiol & Environm Sci, Hangzhou 310058, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Zhejiang Prov Key Lab Plant Virol, Minist Agr,Key Lab Biotechnol Plant Protect,Inst, Hangzhou 310021, Zhejiang, Peoples R China
3.Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Shandong, Peoples R China
关键词： Wheat yellow mosaic virus; Membranous body; Endoplasmic reticulum; Three-dimensional structure
期刊名称：MICRON （ 影响因子：2.251； 五年影响因子：1.969 ）
ISSN： 0968-4328
年卷期： 2019 年 120 卷
页码：
收录情况： SCI
摘要： Plant virus was a kind of organism lived depending on infecting viable host cell and propagated their posterity by replicating its hereditary nucleotide, transcripting into protein, assembling protein and nucleotide into virion (Ortin and Parra, 2006; Sanfacon, 2005). Viral infection usually induces remodeling of host cell, especially endoplasmic reticulum (ER) for generating membrane packed viral factory. During the infection of Bymovirus, a kind of membranous body (MB) was generated in host cells, which is thought as an ER aggregate. In present study we performed a study on Wheat yellow mosaic virus (WYMV) induced MB by several transmission electron microscopy (TEM) based methods, including cytological observation, component analysis by immuno-gold labeling and structural analysis by electron tomography (ET). WYMV infection induced at least two morphologies of MB, including the lamella dominated morphology (lamella-MB) looked like sprawling cirrus, and the tubule dominated morphology (tubule-MB) looked like latticed network. MB was verified composing of ER as revealed by immuno-gold labeling by antibody against endoplasmic reticulum (ER) retention signal as well as by detailed observation of MB construction modules as double layer membrane. By immuno-gold labeling, both two MB morphologies (lamella-MB and tubule-MB) had same components in viral derived protein and membrane origination (from ER). Structural analysis by ET reconstruction revealed the organization of ER in MB. Lamella-MB was composed of cesER like structures arranged irregularly whereas tubule-MB was composed of tubER like structures arranged regularly. This study provided insights into the structural details in how Bymovirus utilizing host membrane system.
- 相关文献
作者其他论文 更多>>
-
Geographical origin verification of Crocus sativus L. grown using "two-segment" cultivation
作者：Tian, Jing;Yuan, Yuwei;Tian, Jing;Li, Chunlin;Shao, Shengzhi;Zhu, Sibei;Mei, Hanyi;Zhang, Yongzhi;Rogers, Karyne M.;Nie, Jing;Yuan, Yuwei;Yang, Jian;Tian, Jing;Li, Chunlin;Shao, Shengzhi;Mei, Hanyi;Zhang, Yongzhi;Rogers, Karyne M.;Nie, Jing;Yuan, Yuwei;Tian, Jing;Li, Chunlin;Shao, Shengzhi;Zhu, Sibei;Mei, Hanyi;Zhang, Yongzhi;Rogers, Karyne M.;Nie, Jing;Yuan, Yuwei;Li, Yuan;Jiang, Chenzhou;Zhu, Sibei;Rogers, Karyne M.
关键词：Crocus sativus L.; Stable isotopes; Two -segment cropping system; Geographical origin
-
Fast prediction of diverse rare ginsenoside contents in Panax ginseng through hyperspectral imaging assisted with the temporal convolutional network-attention mechanism (TCNA) deep learning
作者：Wang, Youyou;Wang, Siman;Bai, Ruibin;Wan, Xiufu;Nan, Tiegui;Yang, Jian;Huang, Luqi;Yuan, Yuwei;Li, Xiaoyong;Li, Xiaoyong;Li, Xiaoyong;Bai, Ruibin;Yang, Jian
关键词：Hyperspectral imaging; Panax ginseng; Rare ginsenoside; Temporal convolutional network -attention; mechanism (TCNA) model; Simultaneous prediction
-
Prediction performance and reliability evaluation of three ginsenosides in Panax ginseng using hyperspectral imaging combined with a novel ensemble chemometric model
作者：Wang, Youyou;Wang, Siman;Bai, Ruibin;Nan, Tiegui;Kang, Chuanzhi;Yang, Jian;Huang, Luqi;Li, Xiaoyong;Li, Xiaoyong;Yuan, Yuwei;Bai, Ruibin;Kang, Chuanzhi;Yang, Jian
关键词：Panax ginseng; Ginsenosides content; Hyperspectral imaging; Deep learning; Uncertainty evaluation; Effective wavelength
-
Unveiling the spatial distribution and molecular mechanisms of terpenoid biosynthesis in Salvia miltiorrhiza and S. grandifolia using multi-omics and DESI-MSI
作者：Xia, Jie;Lou, Ganggui;Qi, Zhechen;Zhang, Xiaodan;Liang, Zongsuo;Yang, Dongfeng;Huang, Yanbo;Yang, Jian;Guo, Juan;Li, Zhenhao;Zhang, Guoliang;Xu, Shengchun;Song, Xijiao;Wei, Yukun
关键词：
-
Combining stable isotope, multielement and untargeted metabolomics with chemometrics to discriminate the geographical origins of ginger (Zingiber officinale Roscoe)
作者：Yu, Dai-xin;Guo, Sheng;Yan, Hui;Wang, Jie-mei;Zhou, Jia-qi;Duan, Jin-ao;Zhang, Xia;Mao, Su-wan;Yang, Jian;Yuan, Yu-wei
关键词：Ginger; Stable isotopes; Multielements; Metabolomic; Chemometrics; Geographical origin
-
A fijiviral nonstructural protein triggers cell death in plant and bacterial cells via its transmembrane domain
作者：Yuan, Zhengjie;Geng, Yanfei;Dai, Yuanxing;Li, Jing;Lv, Mingfang;Zhang, Heng-Mu;Dai, Yuanxing;Liao, Qiansheng;Xie, Li
关键词：cell death; fijivirus; transmembrane domain; viral nonstructural protein
-
Application of hyperspectral imaging assisted with integrated deep learning approaches in identifying geographical origins and predicting nutrient contents of Coix seeds
作者：Wang, Youyou;Xiong, Feng;Zhang, Yue;Wang, Siman;Nan, Tiegui;Huang, Luqi;Yang, Jian;Yuan, Yuwei;Nie, Jing;Lu, Cuncun;Yang, Bin
关键词：Coix seed; Hyperspectral imaging; Geographical origin; Nutrient content; Deep learning; Effective wavelength
