Volume electron microscopy reconstruction uncovers a physical barrier that limits virus to phloem
文献类型： 外文期刊
作者： Lv, Mingfang 1 ; Dai, Yuanxing 1 ; Xie, Li 3 ; Guo, Jiansheng 5 ; Liao, Zhenfeng 7 ; Shang, Weina 3 ; Zhao, Xiaohuan 3 ; Hong, Jian 3 ; Zhang, Heng-Mu 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Peoples R China
2.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China
3.Zhejiang Univ, Anal Ctr Agrobiol & Environm Sci, Hangzhou 310058, Peoples R China
4.Zhejiang Univ, Coll Agr Sci, Hangzhou 310058, Peoples R China
5.Zhejiang Univ, Sch Med, Dept Biophys, Hangzhou 310058, Peoples R China
6.Zhejiang Univ, Ctr Cryo Electron Microscopy, Hangzhou 310058, Peoples R China
7.Zhejiang Acad Agr Sci, Publ Lab, Hangzhou 310021, Peoples R China
关键词： flexible gateway (FL); phloem limitation; plasmodesmal connection; size-dependent physical barrier; virus-induced tumors
期刊名称：NEW PHYTOLOGIST （ 影响因子：9.4； 五年影响因子：10.5 ）
ISSN： 0028-646X
年卷期： 2023 年
页码：
收录情况： SCI
摘要： center dot Most plant reoviruses are phloem-limited, but the mechanism has remained unknown for more than half a century. Southern rice black-streaked dwarf virus (Fijivirus, Reoviridae) causes phloem-derived tumors, where its virions, genomes, and proteins accumulate, and it was used as a model to explore how its host plant limits the virus within its phloem.center dot High-throughput volume electron microscopy revealed that only sieve plate pores and flexible gateways rather than plasmodesmata had a sufficiently large size exclusion limit (SEL) to accommodate virions and potentially serve as pathways of virion movement.center dot The large SEL gateways were enriched within the proliferated sieve element (SE) layers of tumors. The lack of such connections out of the SE-enriched regions of tumors defined a size-dependent physical barrier to high flux transportation of virions.center dot A working model is proposed to demonstrate the mechanism underlying limitation of virus within phloem.
- 相关文献
作者其他论文 更多>>
-
Microscopic probing into plasmodesmata (PD) and PD-derived intercellular gateways (PdIGs): Beyond morphology
作者：Lv, Ming-fang;Dai, Yuan-xing;Zhang, Heng-Mu;Shang, Wei-na;Li, Jun-ying;Li, Yi;Hong, Jian;Xie, Li;Dai, Yuan-xing;Zhang, Heng-Mu;Fu, Shuai;Hong, Jian;Xie, Li
关键词：Plasmodesmata (PD); PD-derived intercellular gateway (PdIG); Intercellular transportation; Microscopy
-
Mitochondrial dynamics imbalance and energy dyshomeostasis mediated developmental toxicity of ochratoxin a in zebrafish (Danio rerio)
作者：Li, Meng;He, Mengai;Liao, Zhenfeng;Li, Chenxi;Mi, Yingqiu;Pan, Ningning;Xie, Xinyi;Liu, Zhiyu;Hou, Jiayin;Chen, Liezhong;He, Mengai;Mi, Yingqiu;Yu, Xueli
关键词：Ochratoxin A; Mitochondrial dynamics; Energy metabolism; Developmental toxicity; Zebrafish
-
Geminiviral replication enhancer hijacks a negative regulator of cytokinin signaling to facilitate viral infection
作者：Guo, Liu-Ming;Li, Jing;Qi, Pan-Pan;Zhang, Heng-Mu;Guo, Liu-Ming;Qing, Ling;Qing, Ling;Qing, Ling
关键词：viral replication enhancer; cytokinin signaling; CK; TATA-box binding protein-associated factor 12b; TAF12b; TAF12b-KMD-RR module; geminiviruses; negative regulator of CK signaling
-
The TATA-box binding protein-associated factor TAF12b facilitates the degradation of type B response regulators to negatively regulate cytokinin signaling
作者：Guo, Liu-Ming;Ghanem, Hussein;Qing, Ling;Guo, Liu-Ming;Li, Jing;Qi, Pan-Pan;Wang, Jie-Bing;Zhang, Heng-Mu;Wang, Jie-Bing;Qing, Ling;Qing, Ling
关键词：TATA-box binding protein-associated factor 12b; TBP TAF12b; cytokinin signaling; CK signaling; type; B response regulators; B-RRs; proteasomal degradation
-
A fijiviral nonstructural protein triggers cell death in plant and bacterial cells via its transmembrane domain
作者：Yuan, Zhengjie;Geng, Yanfei;Dai, Yuanxing;Li, Jing;Lv, Mingfang;Zhang, Heng-Mu;Dai, Yuanxing;Liao, Qiansheng;Xie, Li
关键词：cell death; fijivirus; transmembrane domain; viral nonstructural protein
-
Transcriptomic and Metabolomic Investigation on Leaf Necrosis Induced by ZmWus2 Transient Overexpression in Nicotiana benthamiana
作者：Zhang, Xianwen;Luo, Biao;Wang, Fang;Wang, Yan;Li, Wanchang;Liang, Shuang;Zhou, Zhongjing;Bao, Jiandong;Song, Xijiao;Liao, Zhenfeng;Chen, Guang;Xu, Fei;Teng, Yi;Xu, Shengchun;Lin, Fu-Cheng;Luo, Biao;Bao, Jiandong;Lin, Fu-Cheng;Fang, Ruiqiu;Xu, Shengchun
关键词：WUSCHEL; necrosis; transcriptomics; metabolomics; tobacco; plant transgenic technology
-
A new demethylase gene, OsDML4, is involved in high temperature-increased grain chalkiness in rice
作者：Yan, Yan;Li, Chao;Zhuang, Jun-Jie;Kong, Jia-Rui;Yang, Zhen-Kun;Yu, Jie;Alam, Mohammad Shah;Ruan, Cheng-Cheng;Xu, Jian-Hong;Yan, Yan;Zhang, Heng-Mu;Li, Chao;Xu, Jian-Hong;Liu, Zhen;Yu, Jie;Xu, Jian-Hong
关键词：DNA demethylation; grain chalkiness; high temperature; protein body; seed storage protein; rice (Oryza sativa L)
