Identification and Evaluation of Reference Genes for qRT-PCR Normalization in Sparassis latifolia (Agaricomycetes)
文献类型： 外文期刊
作者： Yang, Chi 1 ; Ma, Lu 1 ; Xiao, Donglai 1 ; Ying, Zhenghe 1 ; Jiang, Xiaoling 1 ; Lin, Yanquan 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Edible Fungi, Fuzhou 350014, Fujian, Peoples R China; Fujian Acad Agr Sci, Natl & Local Joint Engn Res Ctr Breeding & Cultiv, Fuzhou, Fujian, Peoples R China
关键词： normalization; real-time quantitative polymerase chain reaction; reference genes; Sparassis latifolia; light; edible and medicinal mushrooms
期刊名称：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS （ 影响因子：1.921； 五年影响因子：1.879 ）
ISSN： 1521-9437
年卷期： 2019 年 21 卷 3 期
页码：
收录情况： SCI
摘要： Real-time quantitative polymerase chain reaction (qRT-PCR) has emerged as a powerful and popular tool for quantitating differences in transcriptional gene expression levels between samples. Validation of the stability of reference genes is a fundamental step before initiating qRT-PCR assays. Sparassis latifolia is an edible and medicinal fungus containing a remarkably high concentration of beta-glucan, which has many biological and pharmacologic activities. S. latifolia may be a model species for studying fungal photobiology because its fruiting body formation requires more light than other fungi. I Iowever, suitable reference genes for qRT-PCR have not yet been determined. In the present study, 10 candidate reference genes in S. kitffolia were evaluated and validated under different developmental stages and light conditions. To evaluate the suitability of candidate reference genes, three popular software programs (geNonn, NonnFinder, and BestKeeper), along with the delta Ct method, were used to analyze these genes; the final ranking was determined using RefFinder. According to our results, Actin and GAPDH were expressed at the most stable levels under different developmental stages and light conditions.
- 相关文献
作者其他论文 更多>>
-
Long non-coding RNAs and their potential function in response to postharvest senescence of Sparassis latifolia during cold storage
作者：Weng, Mengting;Zhang, Di;Wang, Hongyu;Yang, Chi;Lin, Hongyi;Lin, Yanquan;Weng, Mengting;Zhang, Di;Wang, Hongyu;Yang, Chi;Lin, Hongyi;Lin, Yanquan;Pan, Yanfang
关键词：
-
Chromosome-level assembly of Dictyophora rubrovolvata genome using third-generation DNA sequencing and Hi-C analysis
作者：Ma, Lu;Yang, Chi;Xiao, Donglai;Liu, Xiaoyu;Jiang, Xiaoling;Lin, Hui;Ying, Zhenghe;Lin, Yanquan;Ma, Lu;Yang, Chi;Xiao, Donglai;Liu, Xiaoyu;Jiang, Xiaoling;Lin, Hui;Ying, Zhenghe;Lin, Yanquan;Lin, Yanquan
关键词：Dictyophora rubrovolvata; whole-genome sequencing; functional annotation; lignocellulose degradation; CAZymes
-
Transcriptomic and Metabolomic Profiles Provide Insights into the Red-Stipe Symptom of Morel Fruiting Bodies
作者：Yang, Chi;Jiang, Xiaoling;Ma, Lu;Xiao, Donglai;Liu, Xiaoyu;Ying, Zhenghe;Li, Yaru;Lin, Yanquan
关键词：Morchella; red-stipe symptom; transcriptome; metabolome; tyrosine pathway
-
Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia
作者：Yang, Chi;Ma, Lu;Xiao, Donglai;Liu, Xiaoyu;Jiang, Xiaoling;Lin, Yanquan;Yang, Chi;Ma, Lu;Xiao, Donglai;Liu, Xiaoyu;Jiang, Xiaoling;Lin, Yanquan
关键词：
-
Chromosome-scale assembly of the Sparassis latifolia genome obtained using long-read and Hi-C sequencing
作者：Yang, Chi;Ma, Lu;Xiao, Donglai;Liu, Xiaoyu;Jiang, Xiaoling;Ying, Zhenghe;Lin, Yanquan;Yang, Chi;Ma, Lu;Xiao, Donglai;Liu, Xiaoyu;Jiang, Xiaoling;Ying, Zhenghe;Lin, Yanquan
关键词：Sparassis latifolia; genome; Hi-C sequencing; Oxford Nanopore sequencing
-
Integration of ATAC-Seq and RNA-Seq Identifies Key Genes in Light-Induced Primordia Formation of Sparassis latifolia
作者：Yang, Chi;Ma, Lu;Xiao, Donglai;Ying, Zhenghe;Jiang, Xiaoling;Lin, Yanquan;Yang, Chi;Ma, Lu;Xiao, Donglai;Ying, Zhenghe;Jiang, Xiaoling;Lin, Yanquan
关键词：Sparassis latifolia; ATAC-seq; RNA-seq; light; primordia formation
-
Sequence Analysis and Expression of a Blue-light Photoreceptor Gene, Slwc-1 from the Cauliflower Mushroom Sparassis latifolia
作者：Yang, Chi;Ma, Lu;Ying, Zhenghe;Jiang, Xiaoling;Lin, Yanquan;Yang, Chi;Ma, Lu;Ying, Zhenghe;Jiang, Xiaoling;Lin, Yanquan
关键词：
