Effect of Deep Eutectic Mixtures in Hydroxylation of Fatty Acids: A Correlation between Water Activity and Thermostability of FA-HY1
文献类型： 外文期刊
作者： Zhou, Pengfei 1 ; Zhang, Yan 2 ; Liu, Weiqi 1 ; Jones, Nykola C. 3 ; Hoffmann, Soren Vronning 3 ; Eser, Bekir Engin 2 ; Zhang, Mingliang 2 ; Ouyang, Yi 2 ; Wang, Fang 2 ; Deng, Yuanyuan 1 ; Zhang, Mingwei 1 ; Guo, Zheng 2 ;
作者机构： 1.Guangdong Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Funct Foods, Guangdong Key Lab Agr Prod Proc,Sericultural & Agr, Guangzhou 510610, Peoples R China
2.Aarhus Univ, Dept Biol & Chem Engn, DK-8000 Aarhus, Denmark
3.Aarhus Univ, Dept Phys & Astron, ISA, DK-8000 Aarhus, Denmark
4.Fujian Normal Univ, Coll Life Sci, Fuzhou 350117, Peoples R China
5.Jilin Univ, Sch Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, R China, Changchun 130021, Peoples R China
6.Food Lab Zhongyuan, Luohe 462300, Henan, Peoples R China
关键词： deep eutectic solvents; fatty acid hydroxylation; hydratase; wateractivity; thermostability
期刊名称：ACS SUSTAINABLE CHEMISTRY & ENGINEERING （ 影响因子：8.4； 五年影响因子：8.7 ）
ISSN： 2168-0485
年卷期： 2024 年 12 卷 5 期
页码：
收录情况： SCI
摘要： To validate the performance and application of fatty acid hydratases in green and sustainable deep eutectic solvents (DESs), a robust system with the combination of fatty acid hydratase-1 (FA-HY1) from Lactobacillus acidophilus and DESs for the biocatalytic hydroxylation of fatty acids was developed. As the cosubstrate of hydroxylation reactions, water molecules have been proven to be a key factor for the thermostability of FA-HY1 in DES systems. We found that FA-HY1 displayed improved thermostability at lower water activity. In particular, the half-life time of FA-HY1 increased 6-fold in a choline chloride/sorbitol system with water activity (a w = 0.84) compared to an aqueous buffer system. Moreover, the thresholds of a w for regulating the synthesis of hydroxy fatty acids (HFAs) in FA-HY1/DES systems were determined. We further investigated the recyclability of FA-HY1 in a choline chloride/sorbitol system, where significantly, after three rounds of recycling, a high hydroxylation efficiency of 83.2% was still observed. Monitoring the secondary structure of FA-HY1 using synchrotron radiation circular dichroism analysis revealed that the DESs appear to delay the change in confirmation of FA-HY1. In addition, molecular dynamics simulations were performed in DES and aqueous systems, which revealed the mechanistic features of the thermostability of FA-HY1 in DESs.
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