您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Rapid authentication of mutton products by recombinase polymerase amplification coupled with lateral flow dipsticks

文献类型： 外文期刊

作者： Li, Tingting 1 ; Jalbani, Yar Muhammad 1 ; Zhang, Guilan 1 ; Zhao, Zhiyong 2 ; Wang, Zhiying 1 ; Zhao, Yan 1 ; Zhao, X 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Zhongguancun South St 12, Beijing 100081, Peoples R China

2.Shanghai Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Shanghai 201403, Peoples R China

关键词： Mutton fraud; Authentication; Recombinase polymerase amplification; Lateral flow dipstick

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

ISSN： 0925-4005

年卷期： 2019 年 290 卷

页码：

收录情况： SCI

摘要： Shish kebabs and mutton slices are two of the most favorite foods in China, which are often adulterated with low-cost animal meat such as chicken or duck meat. Currently, many methods have been applied to mutton adulteration detection. However, existing methods based on protein or polymerase chain reaction (PCR) are timec-onsuming and tedious, making them unfit for rapid and on-site detection of counterfeit mutton products in restaurants. In this study, a visual lateral flow dipstick (LFD) method in combination with recombinase polymerase amplification (RPA) was developed for authentication of mutton slices or heat-processed shish kebabs. In this method, sheep-specific RPA primer was designed against the cytochrome b (Cyt b) gene. RPA reaction could be performed under 37-42 degrees C and finished within 15 min. Then, the products were added onto the LFD sample pad until the final results could be observed by naked eyes within 5 min. The total time for authenticating mutton products was only about 30 min, including 5 min for DNA extraction. In addition, only a portable and inexpensive dry bath incubator was required for the authentication. The proposed method was therefore a simple and fast approach, which was also specific and sensitive, and it could be used for on-site mutton product fraud detection by food safety inspection authorities.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台