Extraction of polysaccharides under vacuum condition from Lentinus edodes stipe and their antioxidant activities in vitro
文献类型： 外文期刊
作者： Li, Shunfeng 1 ; Wang, Anjian 1 ; Liu, Lina 1 ; Tian, Guangrui 1 ; Xu, Fangfang 1 ;
作者机构： 1.Henan Acad Agr Sci, Inst Agroprod Proc, 116 Huayuan Rd, Zhengzhou 450002, Henan, Peoples R China
关键词： Lentinus edodes stipe; Polysaccharide; Vacuum; Extraction; Antioxidant activity
期刊名称：FOOD SCIENCE AND BIOTECHNOLOGY （ 影响因子：2.391； 五年影响因子：2.108 ）
ISSN： 1226-7708
年卷期： 2019 年 28 卷 3 期
页码：
收录情况： SCI
摘要： To improve the efficiency of polysaccharide extraction, vacuum technology was used to breakdown the cell wall to extract polysaccharides from Lentinus edodes stipe, and the optimal parameters were optimized by response surface methodology. Then the antioxidant activities in vitro of the polysaccharides were evaluated. Results showed that the optimal conditions for polysaccharide extraction were temperature of 62 degrees C, vacuum of 0.08MPa, stirring speed of 1200r/min, time of 25min and material/liquid ratio of 1:26 (g/mL). Under these conditions, the yield of polysaccharide was 4.28%. The decolorization rate and deproteinization rate of polysaccharides treated by kaolin and hydrogen peroxide were higher than that of activated carbon. However, there was no significant influence on the polysaccharide retention rate, that was about 80%. Results also indicated that polysaccharide treated with activated carbon could obtain higher antioxidant activity.
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