Antibody-free colorimetric detection of chlorothalonil in cucumber via the inhibition of an enzyme-triggered reaction
文献类型： 外文期刊
作者： Liu, Qingju 1 ; Han, Ping 1 ; Wang, Hui 1 ; Gong, Wenwen 1 ; Feng, Xiaoyuan 1 ;
作者机构： 1.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China
2.Minist Agr, Risk Assessment Lab Agroprod Beijing, Beijing 100097, Peoples R China
期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）
ISSN： 2046-2069
年卷期： 2019 年 9 卷 18 期
页码：
收录情况： SCI
摘要： Currently known rapid determination of fungicides usually relies on antibody-based immunoassay. This paper reports a simple antibody-free colorimetric assay for chlorothalonil via the inhibition of an enzyme-triggered reaction. The enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase was significantly inhibited by chlorothalonil, and the color change of NBT-PMS system induced from NADH formation was suppressed, which could be used indirectly to assay chlorothalonil. The limit of detection (LOD) was 0.05 M with a linear range from 0.5 to 10 M, and the detection of 1 M chlorothalonil in solution was achieved with a naked-eye readout. In addition, the colorimetric measurement results of the cucumber samples showed a good recovery rate, although the sensitivity was less effective than the instrumental method. Nevertheless, the results demonstrates that the chlorometric method provides potential opportunities for reliable, cost-effective quantitative detection for chlorothalonil residues in vegetables.
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