Identification and expression profiling analysis of NBS-LRR genes involved in Fusarium oxysporum f.sp. conglutinans resistance in cabbage
文献类型： 外文期刊
作者： Liu, Zeci 1 ; Xie, Jianming 1 ; Wang, Huiping 2 ; Zhong, Xionghui 2 ; Li, Hailong 2 ; Yu, Jihua 1 ; Kang, Jungen 2 ;
作者机构： 1.Gansu Agr Univ, Coll Hort, Lanzhou 730070, Gansu, Peoples R China
2.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
关键词： Brassica oleracea; Resistance gene; Bioinformatics; Gene expression; Gene evolution
期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）
ISSN： 2190-572X
年卷期： 2019 年 9 卷 5 期
页码：
收录情况： SCI
摘要： As one of the most important resistance (R) gene families in plants, the NBS-LRR genes, encoding proteins with nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains, play significant roles in resisting pathogens. The published genomic data for cabbage (Brassica oleracea L.) provide valuable data to identify and characterize the genomic organization of cabbage NBS-LRR genes. Ultimately, we identified 105 TIR (N-terminal Toll/interleukin-1 receptor)-NBS-LRR (TNL) genes and 33 CC (coiled-coil)-NBS-LRR (CNL) genes. Further research indicated that 50.7% of the 138 NBS-LRR genes exist in 27 clusters and there are large differences among the gene structures and protein characteristics. Conserved motif and phylogenetic analysis showed that the structures of TNLs and CNLs were similar, with some differences. These NBS-LRRs are evolved under negative selection and mostly arose from whole-genome duplication events during evolution. Tissue-expression profiling of NBS-LRR genes revealed that 37.1% of the TNL genes are highly or specifically expressed in roots, especially the genes on chromosome 7 (76.5%). Digital gene expression and reverse transcription PCR analyses revealed the expression patterns of the NBS-LRR genes upon challenge by Fusarium oxysporum f.sp. conglutinans: nine genes were upregulated, and five were downregulated. The major resistance gene Foc1 probably works together with the other four genes in the same cluster to resist F. oxysporum infection.
- 相关文献
作者其他论文 更多>>
-
The F-box protein OsFBX156 positively regulates rice defence against the blast fungus Magnaporthe oryzae by mediating ubiquitination-dependent degradation of OsHSP71.1
作者：Zhao, Yudan;Xu, Guojuan;Zhu, Xiaoying;Shi, Yanlong;Wang, Ruyi;Kang, Houxiang;You, Xiaoman;Ning, Yuese;Wang, Xuli;Zhong, Xionghui;Liu, Minghao;Wang, Guo-Liang
关键词：26S proteasome pathway; F-box protein; heat shock protein; Magnaporthe oryzae; Oryza sativa; reactive oxygen species; rice blast
-
Comparative Genomic Analysis of Xanthomonas campestris pv. campestris Isolates BJSJQ20200612 and GSXT20191014 Provides Novel Insights Into Their Genetic Variability and Virulence
作者：Chen, Denghui;Han, Rui;Yue, Xiangqing;Xie, Jianming;Chen, Denghui;Zhong, Xionghui;Cui, Jian;Li, Hailong;Han, Rui;Yue, Xiangqing;Kang, Jungen
关键词：brassica oleracea; black rot; virulence; genomic comparisons; Xanthomonas campestris pv; campestris; IS family transposase; whole-genome sequencing; effectors
-
A multi-head self-attention deep learning approach for detection and recommendation of neuromagnetic high frequency oscillations in epilepsy
作者：Zhao, Xiangyu;Yang, Feng;Zhang, Qiusi;Zhao, Xiangyu;Peng, Xueping;Niu, Ke;Li, Hailong;He, Lili;Wu, Ting;Wu, Ting;Chen, Duo;Ouyang, Menglin;Guo, Jiayang;Guo, Jiayang;Pan, Yijie;Pan, Yijie
关键词：high frequency oscillations (HFOs); magnetoencephalography; MEG; deep learning; multi-head self-attention; HFOs detection; HFOs recommendation
-
Complete mitochondrial genome sequencing and identification of candidate genes responsible for C5-type cytoplasmic male sterility in cabbage (B. oleracea var. capitata)
作者：Zhong, Xionghui;Yue, Xiangqing;Cui, Jian;Han, Rui;Gao, Yi;Kang, Jungen;Yue, Xiangqing;Han, Rui
关键词：cytoplasmic male sterility (CMS); ORF222a; ATP17; mitochondrial genome; tapetal cell
-
Comparative analysis of the complete mitochondrial genome sequences and anther development cytology between maintainer and Ogura-type cytoplasm male-sterile cabbage (B. oleracea Var. capitata)
作者：Zhong, Xionghui;Chen, Denghui;Cui, Jian;Li, Hailong;Huang, Yuxin;Kang, Jungen;Chen, Denghui
关键词：Cytoplasmic male sterility (CMS); orf138a; orf154a; Mitochondrial genome; Tapetal cell
-
Identification and development of a core set of informative genic SNP markers for assaying genetic diversity in Chinese cabbage
作者：Li, Peirong;Su, Tongbing;Yu, Shuancang;Wang, Huiping;Wang, Weihong;Yu, Yangjun;Zhang, Deshuang;Zhao, Xiuyun;Wen, Changlong;Zhang, Fenglan;Li, Peirong;Su, Tongbing;Yu, Shuancang;Wang, Huiping;Wang, Weihong;Yu, Yangjun;Zhang, Deshuang;Zhao, Xiuyun;Wen, Changlong;Zhang, Fenglan;Li, Peirong;Su, Tongbing;Yu, Shuancang;Wang, Huiping;Wang, Weihong;Yu, Yangjun;Zhang, Deshuang;Zhao, Xiuyun;Wen, Changlong;Zhang, Fenglan
关键词：Chinese cabbage; Genetic diversity; Molecular markers; Single nucleotide polymorphism (SNP); KASP assays
-
Natural variation in a calreticulin gene causes reduced resistance to Ca2+ deficiency-induced tipburn in Chinese cabbage (Brassica rapa ssp. pekinensis)
作者：Su, Tongbing;Li, Peirong;Wang, Weihong;Zhao, Xiuyun;Yu, Yangjun;Zhang, Deshuang;Yu, Shuancang;Zhang, Fenglan;Su, Tongbing;Li, Peirong;Wang, Huiping;Wang, Weihong;Zhao, Xiuyun;Yu, Yangjun;Zhang, Deshuang;Yu, Shuancang;Zhang, Fenglan;Su, Tongbing;Li, Peirong;Zhao, Xiuyun;Yu, Yangjun;Zhang, Deshuang;Yu, Shuancang;Zhang, Fenglan;Su, Tongbing;Li, Peirong;Wang, Weihong;Yu, Yangjun;Zhang, Deshuang;Yu, Shuancang;Zhang, Fenglan
关键词：calcium; calreticulin; genome-wide association mapping; quantitative trait locus
