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Pink Bollworm Resistance to Bt Toxin Cry1Ac Associated with an Insertion in Cadherin Exon 20

文献类型： 外文期刊

作者： Wang, Ling 1 ; Ma, Yuemin 3 ; Guo, Xueqin 3 ; Wan, Peng 1 ; Liu, Kaiyu 3 ; Cong, Shengbo 1 ; Wang, Jintao 1 ; Xu, Dong 1 ; X 1 ;

作者机构： 1.Hubei Acad Agr Sci, Key Lab Integrated Pest Management Crops Cent Chi, Hubei Key Lab Crop Dis Insect Pests & Weeds Contr, Minist Agr,Inst Plant Protect & Soil Fertil, Wuhan 430064, Hubei, Peoples R China

2.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

3.Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China

4.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China

5.Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA

关键词： Bt cotton; resistance mechanism; Cry1Ac; Bacillus thuringiensis; Pectinophora gossypiella; genetically engineered crop; transposon

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2019 年 11 卷 4 期

页码：

收录情况： SCI

摘要： Insecticidal proteins from Bacillus thuringiensis (Bt) are widely used to control insect pests, but their efficacy is reduced when pests evolve resistance. We report on a novel allele (r16) of the cadherin gene (PgCad1) in pink bollworm (Pectinophora gossypiella) associated with resistance to Bt toxin Cry1Ac, which is produced by transgenic cotton. The r16 allele isolated from a field population in China has 1545 base pairs of a degenerate transposon inserted in exon 20 of PgCad1, which generates a mis-spliced transcript containing a premature stop codon. A strain homozygous for r16 had 300-fold resistance to Cry1Ac, 2.6-fold cross-resistance to Cry2Ab, and completed its life cycle on transgenic Bt cotton producing Cry1Ac. Inheritance of Cry1Ac resistance was recessive and tightly linked with r16. Compared with transfected insect cells expressing wild-type PgCad1, cells expressing r16 were less susceptible to Cry1Ac. Recombinant cadherin protein was transported to the cell membrane in cells transfected with the wild-type PgCad1 allele, but not in cells transfected with r16. Cadherin occurred on brush border membrane vesicles (BBMVs) in the midgut of susceptible larvae, but not resistant larvae. These results imply that the r16 allele mediates Cry1Ac resistance in pink bollworm by interfering with the localization of cadherin.

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