Development and Validation of Sex-Specific Markers in Pelodiscus Sinensis Using Restriction Site-Associated DNA Sequencing
文献类型： 外文期刊
作者： Liang, Hongwei 1 ; Wang, Lihua 1 ; Sha, Hang 1 ; Zou, Guiwei 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China
2.Chinese Acad Fishery Sci, Minist Agr, Key Lab Aquat Anim Genom, Wuhan 430223, Hubei, Peoples R China
关键词： sex-specific marker; RAD-seq; genome walking; sex identification; Pelodiscus sinensis
期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）
ISSN： 2073-4425
年卷期： 2019 年 10 卷 4 期
页码：
收录情况： SCI
摘要： The sex of an animal influences its economic traits, especially in species displaying sexual dimorphism. The Chinese soft-shelled turtle, Pelodiscus sinensis, is an economically important aquatic species that shows significant male sexual dimorphism, with a large body size, faster growth, a thick and wide calipash, and lower body fat. In this study, ten male and ten female turtles were subjected to restriction site-associated DNA sequencing (RAD-seq) using the Hi-Seq 4000 sequencing platform to isolate female-specific DNA fragments. We identified 5967 bp and 6532 bp fragments using genome walking. Three female-specific markers designed from these two fragments were confirmed to separate the sexes of Pelodiscus sinensis perfectly. One of the female-specific markers showed dosage association in female and male individuals. Individuals from different populations (n = 296) were used to validate that the female-specific markers could identify the genetic sex of Pelodiscus sinensis with 100% accuracy. The results of the present study demonstrated that RAD-seq was useful to develop sex-related markers in animals, and verified that the sex determination system of Pelodiscus sinensis belonged to the ZZ/ZW heterogametic system. Importantly, the developed markers could lead to a method for sex-controlled breeding in the Chinese soft-shelled turtle.
- 相关文献
作者其他论文 更多>>
-
Targeting and editing the second exon of bmp6 gene results in a silver carp with reduced intramuscular bones
作者：Niu, Shuang;Li, Xiaohui;Feng, Cui;Zhang, Zewen;Sha, Hang;Luo, Xiangzhong;Zou, Guiwei;Liang, Hongwei;Liang, Hongwei
关键词：Intermuscular bones; bmp6; Hypophthalmichthys molitrix; Transcriptome analysis
-
Antioxidant Capacity and Disease Resistance Enhanced by Dietary D-Glucuronolactone Supplementation in Chinese Soft-Shelled Turtles (Pelodiscus sinensis)
作者：Zhou, Tong;Xue, Mingyang;Zhou, Yong;Liang, Hongwei;Liu, Wei;Wu, Wenyi;Xue, Mingyang
关键词：aquaculture; D-glucuronolactone; antioxidant; immune response; health;
Pelodiscus sinensis ; feeding trial -
Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts
作者：Li, Hangyu;Liang, Hongwei;Gao, Xiaowen;Zeng, Xiangtong;Zheng, Shuo;Wang, Linlin;Yuan, Faming;Xu, Shaohua;Yin, Zhan;Hu, Guangfu;Liang, Hongwei;Yin, Zhan
关键词：satiety neuropeptide; follicle-stimulating hormone; GnRH3; pituitary; sexual maturity
-
Chromosome-scale genome assembly and annotation of Xenocypris argentea
作者：Wu, Yidi;Sha, Hang;Liang, Hongwei;Liang, Hongwei
关键词：
-
A 5′ Promoter Region SNP in CTSC Leads to Increased Hypoxia Tolerance in Changfeng Silver Carp (Hypophthalmichthys molitrix)
作者：Feng, Nannan;Zhang, Jiquan;Feng, Nannan;Li, Xiaohui;Sha, Hang;Luo, Xiangzhong;Zou, Guiwei;Liang, Hongwei;Liang, Hongwei
关键词：
Hypophthalmichthys molitrix ; hypoxia; SNP;CTSC ; HIF-1 alpha -
METTL14 facilitates the process of sexual reversal via m6A RNA methylation in Pelodiscus sinensis
作者：Zhou, Tong;Chen, Guobin;Cao, Jizeng;Ji, Huizi;Zou, Guiwei;Liang, Hongwei;Cao, Jizeng;Ji, Huizi
关键词：METTL14; Pelodiscus sinensis; RNA methylation; Sexual reversal
-
Effects of Different Carbohydrate Levels in Diets on Growth Performance and Muscle Nutritive Value of Ying Carp and Scattered-Scaled Mirror Carp (Cyprinus carpio)
作者：Xiao, Pengfei;Xiao, Pengfei;Wu, Yunya;Sha, Hang;Luo, Xiangzhong;Zou, Guiwei;Liang, Hongwei
关键词：
