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Influence of organic substrates on nutrient accumulation and proteome changes in tomato-roots

文献类型： 外文期刊

作者： Xing, Jiayi 1 ; Gruda, Nazim 3 ; Xiong, Jing 1 ; Liu, Wei 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Vegetable Res Ctr, Beijing 100097, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Urban Agr North China, Beijing 100097, Peoples R China

3.Univ Bonn, INRES Hort Sci, D-53121 Bonn, Germany

关键词： Organic growing media; Soilless culture; Coir; Peat-vermiculite substrate; Nutrient accumulation; Proteome

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2019 年 252 卷

页码：

收录情况： SCI

摘要： The gradually accumulation of nutrients in organic substrates during growing season may have adverse effects on plant development. This study was conducted to determine the mode of nutrient accumulation and alteration at molecular level induced by the excess nutrient stress in tomato. The ICP-MS was used to analyze mineral ions in root zone solution and nutrients uptake by tomato plants; and iTRAQ-based proteomic analysis was conducted to identify proteome changes in tomato roots. Coir and peat-vermiculite were used in substrate treated experiments. Results showed that the content of K+ was higher in coir than in peat-vermiculite whereas it was the opposite for Ca2+. The concentrations of NO3-, SO42- and Mg2+ were significantly higher in peat-vermiculite and coir substrates than in water culture. The peat-vermiculite substrates generally enhanced Ca uptake but reduced P uptake by plants, when compared to both coir and water culture. Compared to water culture, functional annotation analysis of the root proteome revealed that the excess nutrient accumulation induced complex proteomic alterations involved in mineral ion binding and transport. A total of 358 differentially abundant proteins (DAPS) were identified, including 11 mineral ion binding and transport related proteins, such as calmodulin-like protein and nitrate transporter 3.2 under peat-vermiculite and coir cultivations. RT-qPCR was used to validate nine genes encoding DAPS. We believe that these indicators will contribute to a better control of soilless culture systems and a waste reduction in production of tomatoes.

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