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Transposon insertion causes cadherin mis-splicing and confers resistance to Bt cotton in pink bollworm from China

文献类型： 外文期刊

作者： Wang, Ling 2 ; Wang, Jintao 1 ; Ma, Yuemin 4 ; Wan, Peng 1 ; Liu, Kaiyu 4 ; Cong, Shengbo 1 ; Xiao, Yutao 5 ; Xu, Dong 1 ; W 1 ;

作者机构： 1.Hubei Acad Agr Sci, Key Lab Integrated Pest Management Crops Cent Chi, Hubei Key Lab Crop Dis Insect Pests & Weeds Contr, Minist Agr,Inst Plant Protect & Soil Fertil, Wuhan 430064, Hubei, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Huazhong Agr Univ, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Hubei, Peoples R China

4.Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China

5.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China

6.ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA

7.Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA

期刊名称：SCIENTIFIC REPORTS （ 2020影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2019 年 9 卷

页码：

收录情况： SCI

摘要： Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) are cultivated extensively, but rapid evolution of resistance by pests reduces their efficacy. We report a 3,370-bp insertion in a cadherin gene associated with resistance to Bt toxin Cry1Ac in the pink bollworm (Pectinophora gossypiella), a devastating global cotton pest. We found the allele (r15) harboring this insertion in a field population from China. The insertion is a miniature inverted repeat transposable element (MITE) that contains two additional transposons and produces two mis-spliced transcript variants (r15A and r15B). A strain homozygous for r15 had 290-fold resistance to Cry1Ac, little or no cross-resistance to Cry2Ab, and completed its life cycle on Bt cotton producing Cry1Ac. Inheritance of resistance was recessive and tightly linked with r15. For transformed insect cells, susceptibility to Cry1Ac was greater for cells producing the wild-type cadherin than for cells producing the r15 mutant proteins. Recombinant cadherin protein occurred on the cell surface in cells transformed with the wildtype or r15A sequences, but not in cells transformed with the r15B sequence. The similar resistance of pink bollworm to Cry1Ac in laboratory-and field-selected insects from China, India and the U.S. provides a basis for developing international resistance management practices.

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