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Identification and function analysis of a type 2 diacylglycerol acyltransferase (DGAT2) from the endosperm of coconut (Cocos nucifera L.)

文献类型： 外文期刊

作者： Zheng, Yusheng 1 ; Jin, Yuanhang 1 ; Yuan, Yijun 1 ; Feng, Dan 1 ; Chen, Lizhi 1 ; Li, Dongdong 1 ; Zhou, Peng 2 ;

作者机构： 1.Hainan Univ, Dept Bioengn, Minist Educ, Key Lab Trop Biol Resources, Haikou 570228, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

关键词： Coconut; Lipid synthesis; Diacylglycerol acyltransferase (DGAT); Arabidopsis thaliana; Unsaturated fatty acids

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2019 年 702 卷

页码：

收录情况： SCI

摘要： Coconut (Cocos nucifera L.) is one of the most characteristic plants of tropical areas. Coconut oil and its derivatives have been widely used in various industries. In this paper, a type 2 diacylglycerol acyltransferase (DGAT2), which is one of the key enzymes involved in triacylglycerol (TAG) biosynthesis, was first characterized in coconut pulp (endosperm). The results indicated that CoDGAT2 was highly expressed in coconut pulp approximately 7 months after pollination. The heterologous expression of CoDGAT2 in the mutant yeast H1246 restored TAG biosynthesis in the yeast, which exhibited substrate preference for two unsaturated fatty acids (UFAs), palmitoleic acid (C16:1) and oleic acid (C18:1). Moreover, the seed-specific overexpression of CoDGAT2 in Arabidopsis thaliana led to a significant increase in the linoleic acid (C18:2) content (approximately 6%) compared with that in the wild type. In contrast, the proportions of eicosadienoic acid (C20:1) and arachidic acid (C20:0) were decreased. These results offer new insights on the function of CoDGAT2 in coconut and provide a novel molecular target for lipid genetic modification to change the fatty acid (FA) composition of oils.

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