文献类型： 外文期刊

作者： Yu, R. -R. ¹ ; Liu, W. -M. ¹ ; Zhao, X. -M. ¹ ; Zhang, M. ¹ ; Li, D. -Q. ³ ; Zuber, R. - ⁴ ; Ma, E. -B. ¹ ; Zhu, K. Y. ⁵ ; M ¹ ;

作者机构： 1.Shanxi Univ, Res Inst Appl Biol, 96 Wucheng Rd, Taiyuan 030006, Shanxi, Peoples R China

2.Taiyuan Normal Univ, Dept Biol, Taiyuan, Shanxi, Peoples R China

3.Shanxi Acad Agr Sci, Inst Plant Protect, Taiyuan, Shanxi, Peoples R China

4.Tech Univ Dresden, Angew Zool, Dresden, Germany

5.Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA

6.Univ Cote dAzur, CNRS, INSERM, iBV, Parc Valrose, F-06108 Nice 2, France

关键词： chitin organization; chitosan; chitin deacetylase; Locusta migratoria

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

ISSN： 0962-1075

年卷期： 2019 年 28 卷 3 期

页码：

收录情况： SCI

摘要： Cells produce an extracellular matrix (ECM) with a stereotypic organization that is important for tissue function. The insect cuticle is a layered ECM that mainly consists of the polysaccharide chitin and associated proteins adopting a quasi-crystalline structure. Our understanding of the molecular mechanisms deployed during construction of the highly ordered protein-chitin ECM so far is limited. In this study, we report on the role of the chitin deacetylase 1 (LmCDA1) in the organization of the protein-chitin ECM in the migratory locust Locusta migratoria, and LmCDA1 localizes predominantly to the apical tier of the protein-chitin ECM, but it is also found in lower regions. Reduction of LmCDA1 function correlates with lower amounts of chitin and impedes conversion of chitin to chitosan by deacetylation. Establishment of the quasi-crystalline architecture of the protein-chitin ECM is, however, independent of LmCDA1 activity, but it is dependent on another chitin deacetylase, LmCDA2, which has no detectable effects on chitin deacetylation and, as shown previously, no influence on chitin content. Our data reveal that LmCDA1 and LmCDA2 act in parallel and independently from each other in defining the dimensions of the cuticle. Both enzymes are non-uniformly distributed within the protein-chitin matrix, suggesting a site-autonomous function.