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ChIP-cloning analysis uncovers centromere-specific retrotransposons in Brassica nigra and reveals their rapid diversification in Brassica allotetraploids

文献类型： 外文期刊

作者： Wang, Gui-xiang 1 ; He, Qun-yan 2 ; Zhao, Hong 1 ; Cai, Ze-xi 2 ; Guo, Ning 1 ; Zong, Mei 1 ; Han, Shuo 1 ; Liu, Fan 1 ; Jin 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Vegetable Germplasm Improvement, Key Lab Biol & Genet Improvement Hort Crops North, Beijing Vegetable Res Ctr,Minist Agr, Beijing 100097, Peoples R China

2.China Agr Univ, Natl Maize Improvement Ctr China, Beijing Key Lab Crop Genet Improvement, Beijing 100193, Peoples R China

关键词： Polyploids; Brassica; Centromere; Chromatin immunoprecipitation

期刊名称：CHROMOSOMA （ 影响因子：4.316； 五年影响因子：4.159 ）

ISSN： 0009-5915

年卷期： 2019 年 128 卷 2 期

页码：

收录情况： SCI

摘要： Centromeres are indispensable functional units of chromosomes. The evolutionary mechanisms underlying the rapid evolution of centromeric repeats, especially those following polyploidy, remain unknown. In this study, we isolated centromeric sequences of Brassica nigra, a model diploid progenitor (B genome) of the allopolyploid species B. juncea (AB genome) and B. carinata (BC genome) by chromatin immunoprecipitation of nucleosomes containing the centromere-specific histone CENH3. Sequence analysis detected no centromeric satellite DNAs, and most B. nigra centromeric repeats were found to originate from Tyl/copia-class retrotransposons. In cytological analyses, six of the seven analyzed repeat clusters had no FISH signals in A or C genomes of the related diploid species B. rapa and B. oleracea. Notably, five repeat clusters had FISH signals in both A and B subgenomes in the tetraploid B. juncea. In the tetraploid B. carinata, only CL23 displayed three pairs of signals in terminal or interstitial regions of the C-derived chromosome, and no evidence of colonization of CLs onto C-subgenome centromeres was found in B. carinata. This observation suggests that centromeric repeats spread and proliferated between genomes after polyploidization. CL3 and CRB are likely ancient centromeric sequences arising prior to the divergence of diploid Brassica which have detected signals across the genus. And in allotetraploids B. juncea and B. carinata, the FISH signal intensity of CL3 and CRB differed among subgenomes. We discussed possible mechanisms for centromeric repeat divergence during Brassica speciation and polyploid evolution, thus providing insights into centromeric repeat establishment and targeting.

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