您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Expression profiles of sex-related genes in gonads of genetic male Takifugu rubripes after 17 beta-estradiol immersion

文献类型： 外文期刊

作者： Hu Peng 1 ; Liu Bin 1 ; Ma Qian 1 ; Liu Shufang 1 ; Liu Xinfu 1 ; Zhuang Zhimeng 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266000, Shandong, Peoples R China

3.Funct Lab Marine Biol & Biotechnol, Qingdao 266000, Shandong, Peoples R China

关键词： Takifugu rubripes; ovary-to-testis recovery; sex-related genes; mRNA expression

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

年卷期： 2019 年 37 卷 3 期

页码：

收录情况： SCI

摘要： Estradiol treatment during early life stages of tiger puffer Takilugu rubripes induces feminization in genetic males. However, the ovaries in genetic males may revert to testes once estradiol treatment is halted. Therefore studies should investigate molecular mechanisms underlying ovary-to-testis recovery in genetic males after treatment. In the present study, tiger puffer were exposed to 10, and 100 mu g/L 17 beta-estradiol (E-2) from 15 to 100 days post-hatching (dph), then gonad phenotypes and expression profiles of six sex-related genes (cyp19a, foxl2, dmrt1, amh, sox9a, and sox9b) were characterized after the exposure. Results showed that both 10 and 100 mu g/L E-2, induced ovarian development in genetic males at 100 dph. However, all ovaries induced by 10 mu g/L E-2 first developed into intersexual gonads and subsequently reverted to testes after the exposure. As for treatment of 100 mu g/L E-2, while the rest of the ovaries maintained morphological stability, percentages of intersexual gonads reached 38%-57%, and none were reverted to testes. Increased mRNA levels of cyp19a, foxl2 and sox9b and decreased mRNA levels of dmrt1, amh, and sox9a were observed during the ovarian development in genetic males. While contrary gene expression profiles were detected during ovary-to-testis transformation. The mRNA levels of all the six genes were increased during the development of intersexual gonads. These results indicated that up-regulation of dmrt1, amh and sox9a is associated with initial ovary-to-intersexual transformation, and suppression of-foxl2, cyp19a and sox9b is essential for complete ovary-to-testis recovery in genetic males. This research will help to trace the molecular processes underlying gonadal transformation in teleosts.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台