文献类型： 外文期刊

作者： Zhu, Li ¹ ; Wang, Daoping ² ; Sun, Jiusheng ¹ ; Mu, Yongying ² ; Pu, Weijun ¹ ; Ma, Bo ² ; Ren, Fuli ¹ ; Yan, Wenxiu ² ; Zha ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

3.Xinjiang Acad Agr Sci, Res Inst Soil Fertilizer & Agr Water Conservat, Urumqi 830091, Peoples R China

4.Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

关键词： Albino mutant; Chloroplast; Photosynthesis; Proteomics; Sorghum bicolor

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2019 年 139 卷

页码：

收录情况： SCI

摘要： Leaf color mutants are ideal materials for chioroplast development and photosynthetic mechanism research. Here, we characterized an EMS (ethyl methane sulfonate)-mutagenized sorghum (Sorghum bicolor) mutant, sbe6-a1, in which the severe disruption in chioroplast structure and a chlorophyll deficiency promote an albino leaf phenotype and lead to premature death. The proteomic analyses of mutant and its progenitor wild-type (WT) were performed using a Q Exactive plus Orbitrap mass spectrometer and 4,233 proteins were accurately quantitated. The function analysis showed that most of up-regulated proteins in mutant sbe6-a1 had not been well characterized. GO-enrichment analysis of the differentially abundant proteins (DAPs) showed that up-regulated DAPs were significantly enriched in catabolic process and located in mitochondria, while down regulated DAPs were located in chloroplasts and participated in photosynthesis and some other processes. KEGG pathway-enrichment analyses indicated that the degradation and metabolic pathways of fatty acids, as well as some amino acids and secondary metabolites, were significantly enhanced in the mutant sbe6-a1, while photosynthesis -related pathways, some secondary metabolites' biosynthesis and ribosomal pathways were significantly inhibited. Analysis also shows that some DAPs, such as FBAs, MDHs, PEPC, ATP synthase, CABs, CHLM, PRPs, pathogenesis-related protein, sHSP, ACP2 and AOX may be closely associated with the albino phenotype. Our analysis will promote the understanding of the molecular phenomena that result in plant albino phenotypes.